9). When testing cytotoxicity using a CyQuant assay we found that normalized on the same number of exosomes used per cell (104 exosomes/cell) toxicity of exosomes from 5xFAD brain was 4-fold higher than that from wild type brain and 3.5-fold higher than that from 5xFAD serum exosomes. ...
Dysregulation of hypothalamic ceramides has been associated with disrupted neuronal pathways in control of energy and glucose homeostasis. However, the specific ceramide species promoting neuronal lipotoxicity in obesity have remained obscure. Here, we find increased expression of the C16:0ceramide-producing...
Fig. 1: Distribution of ceramide and dihydroceramide measurements. ADistribution of the absolute (dh)ceramide plasma concentrations; note that thex-axis is log scaled.BComparison of Z-scores derived from the non-transformed and log-transformed (dh)-ceramide plasma concentrations. Cer ceramide, dhCe...
To enhance the aqueous dispersibility of ceramide (Cer) NP, composites were prepared with high amylose maize starch nanoparticles (HSNP) using dry heat treatment. Differential scanning calorimetry results indicated that dry heat treatment at 120 °C for 120 min minimized the recrystallization of Cer ...
NP type C diseases are caused by defects in a protein, NP C1 protein, which is located in membranes inside the cell and is involved in the movement of cholesterol and lipids within cells [281]. A deficiency of this protein leads to the abnormal accumulation of cholesterol and glycolipids in...
Additional studies from this group showed that CerS may be a genetic modifier of CGG toxicity (Kong et al., 2019).The impact of polyglutamine expansions on ceramide metabolism has also been studied in the brains of patients with spinocerebellar ataxia type 2 (SCA2) and in a mouse model, ...
Dysregulation of hypothalamic ceramides has been associated with disrupted neuronal pathways in control of energy and glucose homeostasis. However, the specific ceramide species promoting neuronal lipotoxicity in obesity have remained obscure. Here, we f
NPD type B (NPDB) is a nonneuronopathic type of acid sphingomyelinase (ASM) deficiency. The carrier frequency is 1 in 90 in the Ashkenazi Jewish population. The prevalence is estimated to be 1 in 250,000 in the general population. SMPD1 and NPD1 are the genes associated with NPD. ...
1B), and midbody of dividing NP cells derived from human iPS cells (Fig. 1C). In NP cell–derived neurons, ceramide-containing structures were associated with microtubules (Fig. 1D). The specificity of anti-ceramide IgG was previously confirmed by colabeling of intracellular structures, ...
Activated astrocytes propagate Aβ in extracellular vesicles and increase the toxicity of the peptide to neurons, as well35,43. Here, we observed that activated astrocytes in FAD mice are associated with increased levels of ceramide, and that there is a correlation between densitometric analysis of...