After sterilization, 33.3 mL or 100 mL of the 60 % stock glucose solution (SC-2 or SC-5, respectively), 20 mL of the 50x stock MES buffer solution, and sterile dH2O were added to adjust the total volume to 1.0 L. Yeast Synthetic Drop-out medium supplements (SIGMA) ...
Transganglionic axonal tracers, IB4 (Vector Laboratories, Burlingame, CA) and CTB (List Biological Laboratories, Campbell, CA) were dissolved in sterile phosphate buffered saline (PBS; pH 7.4) for injections (1 ul of 2% solution for each tracer). IB4 was injected into left DCNs and CTB ...
Experience in design and development of supply chain functions at two academic medical centers and one community hospital to include equipment planning, receiving dock, storage/warehousing, PAR unit storage, procedural area storage, sterile processing, equipment management, product flow, and staffing. Sub...
These personnel, however, are more likely to make operational errors owing to their limited experience with the imaging system, potentially requiring the surgeon or their assistant to leave the sterile operating area so that they can operate the computerized imaging systems directly, after which re-...
Packed RBC units, kindly provided by Dr. Tatsuro Yoshida (New Health Sciences, Inc., Cambridge, MA, USA), were collected and stored as previously reported.[18] Samples (0.5 mL) were obtained through sterile couplers at days 2, 7, 14, 21, 28, 35 and 42.[18] RB...
Subsequently, samples were stored in two separate 1.5 mL Eppendorf tubes using sterile pipettes, immediately placed in an insulated box filled with dry ice, and transported to the laboratory for storage at -80 °C before further processing. One sample was used for 16SrRNA sequencing and the ...
Care was taken to ensure each sample was collected in a sterile manner, tools were sur- face sterilized between samples using 95% ethanol and all samples were stored in sterile single use plastic bags. Roots were excavated to a depth of 20–30 cm using a sterilized shovel, and then ...
Thus far, the majority of the genetic factors influencing bacterial colonization have been evaluated in experimental investigations involving a single strain, conducted in sterile environments. However, bacterial colonization can be influenced by both plant–bacteria and bacteria–bacteria interactions. ...
The organisms were transported to land, washed with sterile seawater in an aseptic space, and preserved in cryovials with liquid nitrogen. Each cryovial included a mixture of the pedicellaria, spines, digestive tract, and gonads of its respective replicate. Once in the laboratory, the cryovial...
This has also been shown in the assays carried out as follows: Methods: The 3.3×1010 pfu/ml suspension was either used directly or diluted using sterile saline before injection into the brain of 10 rats. Other methods are as described in Assays 4 except that sections were cut in the ...