Thus far, the majority of the genetic factors influencing bacterial colonization have been evaluated in experimental investigations involving a single strain, conducted in sterile environments. However, bacterial colonization can be influenced by both plant–bacteria and bacteria–bacteria interactions. ...
In all reactions, negative controls were used, which were performed with sterile water to eliminate possible contamination. 2.6. Statistical Analysis of Data Data normality was verified with the Shapiro–Wilk test. According to data distribution, the Mann–Whitney test for non-normal samples was ...
NLRP3 inflammasomes, which were initially studied more in immune cells, have recently been found to play an important role in the sterile inflammation of diabetic kidney disease (DKD) [71]. Under the stimuli of high glucose, ROS and AGEs, the activated NLRP3 inflammasome induces the pro-inflam...
PRRs induce antiviral immune responses by recognizing nucleic acids produced by exogenous pathogens [36,37]. During HSC formation, the ligands for PRRs are puzzling because the AGM region is supposed to be a sterile niche. However, abundant transposable elements ...
(Irvine, CA, USA) in a sterile Falcon tube filled to maximum to avoid aerobic headspace. Samples were frozen at − 20 °C after sampling, then kept at < 7 °C during transport to Montréal where they were again stored at − 20 °C prior to processing. Thermochron ...
In response to a wound, fibroblasts are activated to migrate toward the wound, to proliferate and to contribute to the wound healing process. We hypothesize that changes in pre-mRNA processing occurring as fibroblasts enter the proliferative cell cycle are also important for promoting their migration...
Although the consumer use of formaldehyde at concentrations ≥0.1 % has been prohibited in the EU (23) due to its carcinogenicity, it is still omnipresent in the environment and widely used in resin production, construction, wood processing, textiles, hospitals, laboratorie...
was used for quantification of cells. Enumeration of aerobic and anaerobic heterotrophic cell counts was done by resuspending 0.1 g of each sample in 9 ml sterile sodium pyrophosphate (0.1%w/v), followed by dilution of the extracted liquid up to 10− 4using 0.9% (w/v) sterile saline....