1.A method of promoting regeneration of a lesioned CNS axon of a mature neuron determined to be subject to regeneration inhibition by Smad2/3 signaling, the method comprising the steps of:(a) contacting the neuron with an inhibitor of Smad2/3 signaling sufficient to promote regeneration of the...
Narumiya, et al., [24] Use and Properties of ROCK-Specific Inhibitor Y-27632, Methods Enzymol, 325:273-84, 2000. Ogata, et al., Fasudil Inhibits Lysophosphatidic Acid- Induced Invasiveness of Human Ovarian Cancer Cells, Int J Gynecol Cancer, 19:1473-80, 2009. Ray, Jet al., Fragment...
48 hours after transfection, cells were collected with cold PBS and lysed in 1 ml lysis buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 10% Glycerol and 1% NP-40 supplemented with complete protease inhibitor (Roche). Cells were lysed on ice for 30 min and the whole cell ...
In another aspect of the present invention, the compounds according to the general formula (I) as well as pharmaceutically acceptable salts thereof are used as an inhibitor for a protein kinase, preferably as an inhibitor for a cellular protein kinase....