in tumours is still missing. In this study, we tested whether a widespread tumour microenvironmental condition, low pH, could induce polyploidization in mammalian cells. We found that an acidic microenvironment, in the range of what is commonly observed in tumours, together with the addition of ...
[256] have reported that neutrophils that undergo NETosis in the joints of RA patients can generate active PAD, which can help provide citrullinated autoantigens. RA autoantibodies and other proinflammatory cytokines can cause neutrophils to produce NETs [257]. In blood and synovium samples of RA...
As shown in Figure S1B, after cells entered confluence with this protocol, only a small fraction (2%) of cells undergo DNA replication over a 7 day time course as assessed continuous incubation with EdU. However, to ensure that no mitotic cells were present in the population, the media was...
In any living cells, mitochondria undergo active fusion and fission to keep the integrity of the mitochondrial network. The fidelity of mitochondrial fusion and fission is required to maintain the normal energy production through OXPHOS and TCA cycle. In higher eukaryotes, the most important ...
[ 17 ], and dna repair factors like uracil-dna-glycosylase [ 18 ]. such replication factors undergo dynamic re-distribution in the nucleus during s phase. in the g1 phase, most replication proteins are homogeneously distributed in the nucleoplasm. upon entry into s phase, replication proteins ...
(Fig.7a, Supplementary Data7a, b). Sheih et al. reported that infused CAR-CD8+T cells in vivo undergo early rapid expansion and later stable maintenance40. We found a parallel rapid aging of CAR-CD8+T cells at the expansion phase (28.6 ± 5.8 years) followed by a slowed pace of ...
S4d). A plausible explanation for this discrepancy is that normal cells, as a significant portion in the remission sample, could also undergo cellular senescence in response to chemo-agent-induced stress [47]. Nevertheless, given our small pediatric AML cohort, it remains to be investigated ...
they also express a non-functional truncated p53. Since p53 is involved in DNA damage signalling and directs cells to either arrest at G0/1 to undergo DNA repair, or undergo apoptosis, we considered that our results from experiments using Caco-2 cells could be influenced by these two factors...
Human DCs undergo cell death after infection with live Mtb, in a manner that does not involve executioner caspases, and results in no mycobactericidal effect. Nonetheless, the DC maturation and cytokine profile observed suggests that the infected cells can still contribute to TB immunity. ...
DNA replication and causes a transient, time-dependent increase in the fraction of cells in G2–phase (Fig.1A). This increase reflects predominantly the arrest of cells irradiated in S-phase, as only progression of cells from S-phase can increase the fraction of cells in G2above the levels...