1.Understanding the Cell Cycle: The cell cycle is divided into two main phases: the M phase (mitotic phase) and interphase. Interphase is further divided into three phases: G1, S, and G2. 2.G1 Phase: In the G1 phase, the cell is metabolically active and prepares for DNA replication....
Pulse labeling of asynchronous cells with [ 3H]dThd, continuous labeling of synchronized cells with BrdUrd and prematurely condensed chromosome (PCC) studies of G1 and G2 phase S 18 cells indicate that dm-DNA replicates only once during S phase of the cell cycle. No evidence was found for ...
d-f, Western blots of whole cell extracts from siRNA transfected U-2OS cells. Where applicable cells were treated with 0.4 µM APH for 8 hours. Data are representative of three biological replicates. g, Percentage of F-actin positive S-phase nuclei determined by live imaging of siRNA ...
because we verified microscopically that zygotes showed an expected and consistent spatial pattern of DNA synthesis through S phase (Extended Data Fig.1d,e). To provide a quantitative metric of the RT programme we computed a variability score, which measures the variance of the replication programme...
Chromatin condensation and cessation of transcription are hallmarks of the end of the oocyte growth phase [45]. Many of the DEGs were downregulated in the largest oocyte diameter group (> 120 μm), likely related to the reduction in transcription activity at this stage. Conversely, genes ...
Re-entry into the cell cycle of growth arrested pre-adipocytes is known as the clonal expansion phase and considered to be a prerequisite for terminal differentiation in 3T3-L1 adipocytes [22]. Genes grouped in cluster 2 are highly expressed from 6 hours (onset of clonal expansion) to 3 ...
Here, we investigate the relationship between forespore inflation driven by DNA translocation and the membrane fission event that releases the forespore into the mother cell cytoplasm. We previously showed that this event is catalyzed by the protein FisB that accumulates at the neck of the engulfing...
program, cell cycle dependently expressed genes showed a higher expression independently from the cell cycle phase and a lower amplitude of dynamic changes in cancer cells as compared to untransformed fibroblasts. Contrary to mRNA changes, miRNA expression was stable throughout the cell cycle. ...
The genes in this cluster that were related with GO terms of transcription factor activity, DNA binding and regulation of cell cycle are presented in Supplemental Data Set 2. The set of 114 transcription factors and/or DNA binding proteins were subjected to a secondary GO enrichment analysis to...
The relationship between chromatin organization and gene regulation remains unclear. While disruption of chromatin domains and domain boundaries can lead to misexpression of developmental genes, acute depletion of regulators of genome organization has a