During each cell cycle, the process of DNA replication timing is tightly regulated to ensure the accurate duplication of the genome. The extent and significance of alterations in this process during malignant transformation have not been extensively explored. Here, we assess the impact of altered rep...
000–50,000 SSBs and/or single-strand gaps each S phase. PARP1-dependent signaling and repair may thus help ensuring the integrity of nascent DNA strands during DNA replication10. Consistent with this idea, SSB
We describe a novel 25 kDa protein, geminin, which inhibits DNA replication and is degraded during the mitotic phase of the cell cycle. Geminin has a destruction box sequence and is ubiquitinated anaphase-promoting complex (APC) in vitro. In synchronized HeLa cells, geminin is absent during G1...
These four phases enable exquisite control of DNA replication and cell division cycles and are highly conserved. The main processes during the cell cycle include related cellular functions during each phase, the control of cell cycle checkpoints, replication of cellular components, and mechanisms for ...
interaction; this seems likely as the interaction of theMCPH1tandem BRCT domains with proteins likeE2F1and r-H2AX is required for the activation of cell cycle checkpoint, DNA repair and apoptosis [19–23]. Several studies have likewise suggested thatMCPH1may also function as a tumor suppressor ...
this category for specific common DEGs of ZYDH381–1 and DH3732 (Fig.6e). Of these, cyclin-dependent kinase 12/13 (CDK12_13) belongs to CDKs, which are the core regulators of the cell cycle and are assumed to control cell differentiation and proliferation in response to phytohormone ...
Evidence that F'lac replicates asynchronously during the cell cycle of Escherichia coli B/r. Mol. Gen. Genet. 163:205-212.Andresdottir, V., and M. Masters. 1978. Evidence that F'lac replicates asynchronously during the cell cycle of Escherichia coli. Mol. Gen. Genet. 163:205-212....
The proteins P5 and P10 cause cell lysis and the release of progeny virions [12,22]. The quantitation of the copy number of every protein is important for understanding the assembly and transformations of the virion and its sub-particles during the life cycle [23]. In the following section...
Analysis was conducted with an average of ten independent biological replicates. The relative peak area was normalized by the average value of all samples. Fold changes are visualized using a color gradient from red (high) to blue (low). The principle component analysis (PCA) was used to ...
Cluster 3 indicates that many processes related to cell cycle and DNA repair are strongly induced and maintained from day 3 onward (Figure 2D), including cell cycle proteins such as Cdk1, Cdk2, Cyclin B1, Plk1, and NPAT. The latter protein, required for progression through G1 and S phase...