Alternative splicing is a process that allows individual genes to produce multiple functionally distinct protein isoforms. The production of alternatively spliced isoforms is usually controlled in a cell-type or developmentally regulated manner. Keywords: RNA splicing; intron; gene expression; isoformsdoi:10.1038/npg.els.0000890Juan ...
Figure 3. Cell-Type-Specific Alternative Splicing Translocates Ninein from Centrosome in NPCs to Non-centrosomal Loci in Neurons (A) Impact of 61 cytoskeleton-related and conserved AS events on modular protein domains. AA, amino acids; CT, C terminus. (B) Alternative splicing alters protein doma...
Cell-type-specific alternative splicing governs cell fate in the developing cerebral cortex. Cell 166, 1147–1162 (2016). CAS PubMed PubMed Central Google Scholar Makeyev, E. V., Zhang, J., Carrasco, M. A. & Maniatis, T. The microRNA miR-124 promotes neuronal differentiation by ...
Using 3.5 mo cell-type-specific DEGs, we performed gene set enrichment analysis (GSEA, ToppGene40) to examine whether specific molecular functions, biological processes and/or biochemical pathways were significantly enriched. Collectively, DEG sets from astroglia, oRGs, IPC3, CNs, and INs were sp...
We found that the D2 MSN-specific TF identified here first, Sp9, disrupted D1 MSN-specific gene expression, confirming the functional relevance of these cell type-specific TFs (Figure S6). Finally, we observed that neurons contribute to the brain’s uniquely high-level alternative splicing more...
Given the complexity of higher organisms, the number of genes encoded by their genomes is surprisingly small. Tissue specific regulation of expression and splicing are major factors enhancing the number of the encoded products. Commonly these mechanisms
Alternative pre-mRNA splicing is a critical mechanism that generates multiple mRNA from a single gene, thereby increasing the diversity of the proteome. Recent research has highlighted the significance of specific splicing isoforms in cellular processes, particularly in regulating cell numbers. In this ...
[44] revealed the dynamic process and molecular characteristics of gene expression during spermatogenesis in mice and the specific patterns of alternative splicing through single-cell sequencing technology, as well as the discovery of key regulators for specific stages of male germ cell development. In...
a homogeneous cell population. Instead, differential quantification is obtained when using an indicator variable of cell type as a cell level feature: in this modality (Mode 2-diff), the event-specific effect term quantifies the effect of cell type on splicing variability for the specific event...
Deep sequencing reveals cell-type-specific patterns of single-cell transcriptome variation. Genome Biol. 2015;16:122. Article PubMed PubMed Central Google Scholar Mahata B, Zhang X, Kolodziejczyk AA, Proserpio V, Haim-Vilmovsky L, Taylor AE, et al. Single-cell RNA sequencing reveals T ...