theory has been endorsed by several microbiological evidences. There are supportingproofs that mitochondria andplastidsineukaryotic cellshave come from bacteria (Margulis, 1970). Evolutionary course and diversity of living organisms can also be affected by endosymbiotic gene transfer (Margulis, 2010). As...
T-cell development provides an excellent model system for studying lineage commitment from a multipotent progenitor. The intrathymic development process has been thoroughly studied. The molecular circuitry controlling it has been dissected and the necess
NanoJ-eSRRF, ThunderSTORM and HAWK. To create datasets with increased emitter density, temporal binning was performed by summing substacks of the SMLM image sequence. The raw data had an average emitter density of 0.121 localizations per frame and µm2, which remained constant throughout the wh...
Beyotime, CHN), according to the manufacturer’s protocols. Briefly, Swan 71 cells were incubated with Mito Tracker Red CMXRos dye solution at room temperature for 20 min in dark. The MMP was determined on a fluorescence inverted microscope...
Once it was clarified that oocyte activation is stimulated by Ca2+, the next question that logically occurred was how the sperm triggers the Ca2+elevation in the ooplasm? This issue, however, remained unresolved for a long time. Multiple hypotheses were proposed to explain the generation of the...
established the famous diffraction limit formula in 1874 [7,8]. The Abbe limit set the foundation for modern microscopy by establishing for the first time the theory of image formation and the diffraction limit of optical instruments, which states that the resolution of an optical microscope cannot...
(Fig.5d). Attachment with the pole and reorientation was also observed by confocal live-cell imaging (Fig.5e, Supplementary Movies12–14), showing persistence of the ezrin pole throughout the process. However, rapid reorientation of the pole towards the substrate upon attachment cannot be ...
One of the most well-known theories explaining tissue degeneration and aging upon mitochondrial dysfunction is the free radical theory of aging, which was first proposed by Harman in 1956.40,41,42Mitochondria are one of the major sources of ROS but in particular represent an important cellular targ...
The mother tincture was diluted 1:8000 up to 1:3.2 × 106 (Tiliae) or 1:8 × 106 (Crataegi, Salicis, Populi). Isotonic solvent or cell culture medium alone was used as control. 2.3. Tumor Cell Growth Cell growth was analyzed by a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl...
Similar observations were made by Jiang and coworkers, although upregulation of p53 was required for t-BuOOH-triggered ferroptosis in their cellular model [63]. Taken together, there are a multitude of different ferroptosis activators which initiate ferroptotic cell death through canonical and non-...