Dynamics of antibody binding and complement interactions at the cell surfacedoi:10.1016/B978-0-7204-0623-8.50018-XKarl-Gsta SUNDQVISTDynamic Aspects of Cell Surface Organization
Uptake of each peptide was linearly dependent on its cell surface binding, and affinity is thus important for efficiency. However, the gradients of these linear dependencies varied significantly. Thus each peptide's ability to stimulate uptake once bound to the cell surface is reliant on formation ...
Using CD3, a T-cell marker involved in T-cell activation51, as the model, the antibody binding signals against CD3 of CellSia-P and CellGalNAc-P were similar to those of native cells, as reflected by anti-CD3-FITC staining; and the signals of CellMet-P and CellCho-P showed only a ...
3f). In contrast, isotype-matched antibodies targeting the plasma membrane receptor RANK at the surface of osteoclasts had no effect despite comparable levels of binding between α-La mAb and α-RANK antibody to the surface of synchronized cells following LPC removal (Fig. S5a). While RANKL-...
Staining cell surface targets protocols Protocol A: Cell Suspensions Protocol B: Human Lysed Whole Blood General notes For optimal performance of fluorochrome conjugated antibodies, store vials at 2–8°C in the dark. Do not freeze. Prior to use, quick spin the ant...
Distribution and force spectroscopy of CD20 antigen-antibody binding on the B cell surfaceB 细胞膜CD20 抗原的分布与单分子力谱探测Distribution and force spectroscopy of CD20 antigen-antibody binding on the B cell surfaceB 细胞膜CD20 抗原的分布与单分子力谱探测B-CLL外周...
Compared to proteins, glycans, and lipids, much less is known about RNAs on the cell surface. We develop a series of technologies to test for any nuclear-encoded RNAs that are stably attached to the cell surface and exposed to the extracellular space, he
Cell isolation, also commonly referred to as cell separation or cell sorting, can be achieved using magnetic beads to target cells for selection or depletion by using ligands or antibodies directed against specific cell surface antigens. Labeled cells that are cross-linked to magnetic beads are ...
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A human monoclonal antibody specifically binding to a surface antigen of cancer cell membrane, an isolated DNA encoding the antibody, and a hybridoma producing the antibody. An anti-cancer formulation comprising the monoclonal antibody bonded to the surface of a liposome enclosing an anti-cancer agent...