An improved assay is described where a surface is provided with immobilized anti-Ig antibodies rather than antigen and where specific antibody-secreting cells (ASC) are detected using soluble antigen probes containing one of several possible labels. The method gives improved sensitivity with less back...
3f). In contrast, isotype-matched antibodies targeting the plasma membrane receptor RANK at the surface of osteoclasts had no effect despite comparable levels of binding between α-La mAb and α-RANK antibody to the surface of synchronized cells following LPC removal (Fig. S5a). While RANKL-...
. A proximity barcoding assay using antibody-DNA conjugates has been reported for assessing the composition of surface proteins on individual exosomes[147]. When applied to a large number of exosomes it was reported that “exosomes from different sources are better distinguished when more surface ...
and microtubules were labeled with an anti-ß-tubulin antibody in conjunction with a fluorescein-labeled secondary antibody. Cells vary in width from about 15 µm to about 25 µm. The stages are (from top left): interphase cortical microtubule array; pre-prophase band of microtubules (...
6). Nevertheless, immunofluorescence analyses of paraformaldehyde-fixed cells indicated that EhAct1, EhGrainin1, EhRub and EhURE3-BP were present both inside and on the surface of these cells (Fig. 7). The antibody against EhDNAJ was apparently inadequate for immunofluorescence analysis because ...
The 84-1 “cell-surface vimentin” antibody49, putatively recognizing the beginning of the rod domain50, gave a consistent punctate pattern at the cell surface in non-permeabilized Vero and A549 cells (Fig. 3a). This staining was not homogeneous in the cell population, but clearly distinct fro...
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Assay-dependent - 产品规格 种属反应 Human 宿主/亚型 Mouse / IgG1, kappa 分类 Monoclonal 类型 Antibody 克隆号 CR1/6383 抗原 Recombinant fragment (within aa650-850) of human CD35. 查看免疫原 偶联物 Unconjugated 形式 Liquid 浓度 200 µg/mL ...
cell biolabs MET-5099-5 1-Methyladenosine (m1A) ELISA Kit 5 x 96 assays 6 parts: 5 RT / 1 Blue Ice Upon receipt, aliquot and store the Anti-1-Methyladenosine Antibody and the m1A Standard at -20ºC an ￥31,762.50 cell biolabs MET-5102 5-Formylcytosine (5-fC) ELISA Kit 96 assa...
This areola, or nucleus of the cell, as perhaps it might be termed, is not confined to the epidermis, being also found not only in the pubescence of surface, particularly when jointed, as in Cypripedium, but in many cases in the parenchyma or internal cells of the tissue” [3, 38–40...