An improved assay is described where a surface is provided with immobilized anti-Ig antibodies rather than antigen and where specific antibody-secreting cells (ASC) are detected using soluble antigen probes containing one of several possible labels. The method gives improved sensitivity with less back...
000 ng/ml was also developed. The new cell-binding assay method can be used to efficiently support humanization process for selection of anti-CD20 antibody drug candidates and to characterize antibody binding to other cell surface proteins.
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Antibody 克隆号 84-1 抗原 Human recombinant vimentin 偶联物 APC 查看其它偶联染料 激发/发射光谱 651/660 nm 查看光谱 形式 Liquid 浓度 See Label 纯化类型 Affinity chromatography 保存液 PBS, pH 7.4 内含物 no preservative 保存条件 4° C, store in dark...
MSC nuclei were stained with DAPI (blue), and FITC-labeled secondary antibody exhibited the attachment of C-TMN to the MSC surface (green). (c) Prussian blue staining of rat bone marrow MSCs (BMSCs) treated with UIN or C-TMN showed the binding of C-TMN but not UIN to the MSC ...
(ThermoFisher, Waltham, MA, USA) at room temperature for 30 minutes. The cells were washed twice in 1× PBS by centrifugation at 400 × gfor 5 minutes and stained with the cell surface antibody Sca1-APC (Miltenyi Biotec, Bergisch Gladbach, Germany) for 20 minutes in the dark...
Positive cell isolation involves directly labeling desired cells for selection using an antibody or ligand that targets a specific cell surface protein. Positive cell isolation with Dynabeads magnetic beads In positive cell isolation using Dynabeads magnetic beads, the beads are coated with the specifi...
Cell-surface modification or encapsulation technology was originally developed with microbes such as yeasts [1] andEscherichia coli[2]. Various materials such as silica gel [3], poly(styrene sulfonate sodium salt), poly(allylamine hydrochloride) [1], chitosan, alginate, hyaluronic acid [2], poly...
cell surface proteins such as receptors can be magnetically labelled in two ways: either by a biotinylated ligand in combination with streptavidin-coated magnetic nanobeads (Figure 1A) or by using a specific antibody directed against the receptor to which a secondary anti-isotype-specific antibody, ...
The 84-1 “cell-surface vimentin” antibody49, putatively recognizing the beginning of the rod domain50, gave a consistent punctate pattern at the cell surface in non-permeabilized Vero and A549 cells (Fig. 3a). This staining was not homogeneous in the cell population, but clearly distinct fro...