Investigators were not blinded to allocation during experiments, but outcomes were qualitatively assessed using automated imaging protocols and software. Statistical tests are all two-sided and multiple comparison p-value corrections were applied using an FDR correction....
A major contributing factor to glioblastoma (GBM) development and progression is its ability to evade the immune system by creating an immune-suppressive environment, where GBM-associated myeloid cells, including resident microglia and peripheral monocyte-derived macrophages, play critical pro-tumoral roles...
2023, STAR Protocols Citation Excerpt : All buffers and reagents need to be maintained in a sterile condition. This protocol uses well-characterized iPSCs obtained from healthy donors.2 The protocol can be further extended to patient-derived iPSCs and be adapted to characterize various somatic and...
AIRE is an unconventional transcription factor that enhances the expression of thousands of genes in medullary thymic epithelial cells and promotes clonal deletion or phenotypic diversion of self-reactive T cells1–4. The biological logic of AIRE’s targ
Current β-cell neogenesis protocols involving stem cell differentiation and transformation of non-β-cells to produce β-like cells are inefficient and unstable, and the yield and maturity of the induced-generated β-like cells are still low for clinical needs. Therefore, improving the efficiency ...
single-cell and single-nucleus RNA-seq workflows.Genome Biol.21, 130 (2020).This study compares gene expression and cellular composition of single-cell and single-nucleus suspensions generated implementing different dissociation protocols and different storage methods to identify potential artefacts and ...
All the known scRNA-seq protocols for eukaryotic cells are limited to detection of mRNAs with poly(A) tails (poly(A) + RNAs). There is a substantial amount of non-polyadenylated RNAs (poly(A)-RNAs) expressed in mammalian cells. Priming through oligo(dT) avoids the preponderance of ...
(RNAi) to any intracellular mRNA due to probe internalization, our probe incubation time was limited to 1 h. Such a short incubation time without transfection was in sharp contrast to the large number of published RNAi experiments in monocytes. Monocyte RNAi protocols require overnight ...
I.M. has consulted for Bristol-Myers, Bayer, Roche, AstraZeneca, F-Star, Genmab, Seattle Genetics, Merck Serono, MSD, Alligator and Bioncotech. L.G. provides remunerated consulting to OmniSEQ, Astra Zeneca, Inzen, Boehringer Ingelheim and the Luke Heller TECPR2 Foundation, and he is ...
Chromium single-cell 3′ reagent kits V3 (10X Genomics, Pleasanton, CA, USA) were used for scRNA-seq library preparation according to the manufacturer’s protocols. A single-cell suspension was loaded into the Chromium Controller (10X Genomics) to form single cell GEMs (Gel Bead-in-Emulsions)...