Therapeutic irradiation of the tumor microenvironment causes differential activation of pro-survival and pro-death pathways in malignant, stromal, endothelial and immune cells, hence causing a profound cellular and biological reconfiguration via multiple
Investigators were not blinded to allocation during experiments, but outcomes were qualitatively assessed using automated imaging protocols and software. Statistical tests are all two-sided and multiple comparison p-value corrections were applied using an FDR correction....
Tumor evolution from a single cell into a malignant, heterogeneous tissue remains poorly understood. Here, we profile single-cell transcriptomes of genetically engineered mouse lung tumors at seven stages, from pre-neoplastic hyperplasia to adenocarcinoma. The diversity of transcriptional states increases ...
RNA-seq protocols have been optimized to enable large-scale transcriptional profiling of individual cells. Such single-cell measurements require both improved molecular techniques as well as effective ways to isolate and process a large number of cells in parallel. While single-cell RNA-seq (scRNA-s...
Metabolic engineering projects often require integration of multiple genes in order to control the desired phenotype. However, this often requires iterative rounds of engineering because many current insertion approaches are limited by the size of the DN
Cells were expanded and cultured according to previous validated protocols [20]. For the Ki67 and BIRC3 immunostaining, primary asthmatic BECs (cat#: 00194911) purchased from Lonza Bioscience, Switzerland, were used. Primary cells were seeded in collagen-coated T75 flasks, 6-well culture plates,...
Impact of light quality and sucrose on adventitious shoot regeneration and bioactive compound accumulation in Ajuga multiflora Bunge. Sci. Hort. 2018, 236, 222–228. [Google Scholar] [CrossRef] Ali, H.; Khan, M.A.; Kayani, W.K.; Khan, T.; Mashwani, Z.; Ullah, N.; Raham Sher ...
(Silhavy et al., Experiments with Gene Fusions, Cold Spring Harbor Laboratory Press Cold Spring Harbor, N. Y., 1984); and by Ausubel, F. M. et al., (Ausubel et al., Current Protocols in Molecular Biology, published by Greene Publishing Assoc. and Wiley-Interscience, 1987).Biosynthetic ...
(B) effect on apoptosis, expressed as DNA fragmentation, (C) effect on cortisol production, normalized to cell number, after 6 days of incubation, (D) effect of IFN-β (100 IU/ml; open bars) or mitotane (1 μM; filled bars) on mRNA expression of STAR, CYP11A1, CYP17A1, and...
The flow of traffic can be chaotic, with lit- tle or no adherence to right of way protocols at some in- tersections and liberal use of honking (The Nericell project webpage [11] includes a video clip of a chaotic intersection in Bangalore). Vehicles types are also very heterogeneous, ...