本页是上海玉博生物科技有限公司 为您精选的Cell Signaling Technology, Inc.产品,欢迎您来电咨询该产品的详细信息!上海玉博生物科技有限公司 产品的种类有很多,不同的产品也会有细微的差别,本公司为您提供全方位的解决方案。
Cells were harvested and lysed by adding 1X RIPA buffer (Cell Signaling Technology, Cat no. 9806 S) directly to the culturing plate. RIPA buffer was supplemented with freshly added protease inhibitor cocktail and PMSF with 1:1000 dilution from the original concentration. Cells were collected using...
| carnabio | CaroteNature | Carver Incorporated | cascade bioscience | cayman | cayman chemical | CDN | cedarlane | cedarlanelabs | celartia | celex | cell biolabs | cell marque | Cell Point Scientific | cell projects | cell sciences | cell signaling | cell signaling technology | Cell Signa...
which were purchased from Cell Signaling Technology (CST, Beverly, MA, USA). Bcl-2 (12789-1-AP, 1:1000 dilution), PDI (11245-1-AP, 1:200 dilution), PERK (20582-1-AP, 1:1000 dilution), BiP (11587-1-AP, 1:1000 dilution), eIF2α (11170-1-AP, 1:1000 dilution), ATF4 (10835-...
The next day cells on coverslips were washed with PBS and incubated for 1 h at room temperature in a humid chamber with the secondary antibody: goat anti-mouse conjugated with AlexaFluor 488 (Cell Signaling Technology Inc., Danvers, MA). After this step, the cells were washed again with...
(#3700S, Cell Signaling Technology, 1:1000). In contrast, mouse cell lysates were prepared and their protein concentrations determined using a BCA kit. The primary antibodies for mouse cells were anti-KIT (#PA6364, abmart, Shanghai, China, 1:500) and anti-β-actin (#3700S, Cell ...
Crowberry inactivated the PI3K/Akt signaling pathway by regulating DEK in vitro and significantly inhibited tumor growth by downregulating the DEK expression in xenograft models. Conclusion Crowberry inhibits CCA cells proliferation and migration through a molecular mechanism that includes inhibition of DEK...
The following antibodies were used in this study: anti-GFP antibody (632375, Clontech, Palo Alto, CA), anti-p44/p42 MAPK (ERK1/2) antibody (4695, Cell Signaling Technology, Danvers, MA), anti-RSK2 (sc-9986, Santa Cruz Biotechnology, Santa Cruz, CA), anti-HaloTag antibody (G9281, ...
and they found that TNF and IL-1β were mainly expressed by cytotoxic T cells, NK cells and monocytes respectively. Aside from communicating with myeloid subsets, iMSCs committed with proliferating MM cells via the CCL2-CCR2 signaling pathway. More importantly, they demonstrated that stromal infla...
(No. 4526), anti-phospho-BRCA1 Ser1524 (No. 9009), anti-phospho-Chk1 Ser296 (No. 2349) from Cell Signaling Technology, Inc. (Danvers, MA, USA); and anti-α-tubulin (T6074) from Sigma-Aldrich Srl. Chemiluminescent detection reagent has been purchased from Millipore Spa (Luminata ...