(1:500 dilution, Santa Cruz Biotechnology, USA), anti-β-actin (1:2000 dilution, ZSGB-BIO, China), anti-cleaved caspase-9 (1:500 dilution, Santa Cruz Biotechnology, USA), or anti-cleaved caspase-3 (1:1000 dilution, Cell Signaling Technology Inc, USA) antibodies overnight at 4 °C....
(1:1000, no. 5114, Cell Signaling Technology, Danvers, MA, USA), anti-mTOR (1:1000, no. 2983, Cell Signaling Technology), anti-p-mTOR (1:1000, no. 2971, Cell Signaling Technology), anti-MBNL1 (1:1000, no. sc-47740, Santa Cruz Biotechnology), and anti-tubulin (1:1000, no. ...
Another aspect of expression reconstruction robustness is the dependence of the algorithm on the cell type or cell state similarity of the lq and hq datasets. In the optimal case, DISCERN would not require that the lq and hq datasets have overlapping cell types to perform an accurate expression ...
maturation, and stabilization [132]. The initiation step is characterized by the expression of genes that encode proteins involved in MET via the silencing of SNAIL1/2, suppression of TGF-β signaling, and upregulation of CDH1 [133]. In the maturation step, the expression of...
1F). Compared to the parental cells (T24 and 253J), decreased MARCH7 expression was also observed in spheres, which have cancer stem-like cells (CSCs) properties (Interleukin 6 signaling maintains the stem-like properties of bladder cancer stem cells)(Fig. 1G). Fig. 1 MARCH7 was a ...
In recent decades, many novel non-apoptotic cell death forms, including ferroptosis4, necroptosis5, pyroptosis6, autophagic cell death7, paraptosis, lysosomal-dependent cell death, and oncosis8, have been discovered to reveal different signaling pathways and molecular mechanisms other than apoptosis, ...
ChIP-PCR assay was carried out using the SimpleChIP® Enzymatic Chromatin IP kit (Magnetic Beads) (Cell Signaling Technology, Danvers, MA, USA) by following the manufacturer's procedure. Briefly, the KYSE170 cells were seeded in 15 cm dishes until they grew to more than 90% of confluence...
Journal of Developmental Biology Review The Signaling Network Controlling C. elegans Vulval Cell Fate Patterning Hanna Shin 1 and David J. Reiner 1,2,* 1 Institute of Biosciences and Technology, Texas A&M Health Science Center, Houston, TX 77030, USA; hshin2@medicine.tamhsc.edu 2 College ...
Giα proteins form a complex with RTKs and are important for phosphorylation of Gab1 and Shp2, the association of Gab1 with Shp2, as well as the interaction of Gab1 with Grb2 leading to activation of the ERK1/2 and Akt/mTORC1 signaling pathways. Functional differences among Giα ...
(1:1000), total S6 (1:1000), survivn/BIRC5 (1:1000), pan phospho-PKC (1:1000) (Cell Signaling Technology, Beverly, MA), PAK6 (1:400) (R&D Systems, Minneapolis, MN). Luminescent signal was captured on a Gel Logic 2000 imaging system with Carestream Molecular Imaging software (...