该研究使用了18个Proteintech产品,其中包括ASC/TMS1、Beta Actin、Caspase 1/p20/p10、IkB Alpha、MFN1、MFN2、MYD88、NF-κB p65、OPA1、Pan Acetylation、PHGDH、SIRT5和TLR4 共13个抗体;IL-1(mouse)、IL-10(mouse)和TNF-alpha(mouse) 共3个ELISA Kit;IFN gamma(human)和IL-4(human)共2个HumanKine® ...
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CST原装抗体/Cellsignal抗体,Cell Signaling Technology (CST) 是美国著名的生物公司和细胞信号转导研究的,提供特色的信号转导检测用相关产品,用于细胞功能和疾病发病机理的探讨。产品覆盖肿瘤,糖尿病,神经退行性疾病,干细胞,表观遗传学和免疫学等众多领域!
Total RNA was extracted from cells using TRIzol (Invitrogen). 2 μg of total RNA was then reverse transcribed using the iScript Advanced cDNA kit for RT-qPCR (Bio-Rad), and subjected to real-time PCR using the SYBR Green reagents (Bimake). The sequences of the primers used for real...
心脏内皮细胞用TGF-β受体(TbetaRI)激酶的小分子抑制剂SB431542处理能完全阻断TGF-β2诱导EndMT,但MEK抑制剂PD98059不能。在EndMT成纤维细胞样细胞中,α-SMA,Snail,beta-catenin以及乙酰转移酶P300(ATp300)的转录和蛋白水平明显升高。 重要的是,小分子RNA(miRNA)的相关数据显示,在不同的心血管疾病失调的特定的miRN...
Expression of actin-related genes is controlled by TGFβ signaling To identify the genetic networks regulated by TGFβ signaling in muscle cells, we performed transcriptome analysis of primary myocytes differentiated for 24 h and stimulated with either TGFβ1 or ITD-1 for another 24 h (Supple...
该研究提示可能确实NPC库才是人类大脑进化的关键。beta-catenin除了增强Wnt信号通路外,也是NPC细胞连接的粘附因子之一,稳定beta-catenin理论上也可以延缓NPC向vRG的形态转化。那么抑制形态转化是否也与更大的祖细胞库和更宽的皮层相关,目前尚未知。 2021年3月24日,Cell杂志在线发表了英国剑桥MRC分子生物学实验室Madeline ...
9321S Phospho-Tyk2 (Tyr1054/1055) Antibody, 100 ul KIT Cell Signaling(CST) 9322S Phospho-GSK-3-beta (Ser9) (D3A4) Rabbit mAb, 100 ul KIT Cell Signaling(CST) 9323P Phospho-GSK-3B (Ser9) (5B3) Rabbit mAb, 40 ul KIT Cell Signaling(CST) 9323S Phospho-GSK-3B (Ser9) (5B3...
2020年6月25日来自德国波恩大学药理毒理研究所的AlexanderPfeifer教授团队在Cell Metabolism上发表题为Adenosine/A2B Receptor Signaling Amelioratesthe Effects of Aging and Counteracts Obesity的文章。 研究人员表明,腺苷A2B受体(A2B)在骨骼肌(SKM)...
(26632) were purchased from Cell Signaling Technology (Beverly, MA). Polyclonal anti-GABARAPL1 (11010-1-AP) was from Proteintech Group (Chicago, IL). Anti-ERα (sc-8002), anti-ERβ (sc-373853 X) and anti-β-Actin (sc-47778) were from Santa Cruz Biotechnology (Santa Cruz, CA). Anti...