**Po0.01, ***Po0.001 Santa Cruz, CA, USA, p-c-Jun, p-JNK, p-eIF2, cleaved caspase-3, b-actin (Cell Signaling), DP5 (AbD Serotec, Oxford, UK) and a-tubulin (Sigma). The proteins were detected using the corresponding horseradish peroxidase-conjugated secondary antibody (Santa Cruz ...
针对聚集蛋白、II型胶原蛋白、MMP-13、HO-1、ADAMTS5、b-actin、诱导型一氧化氮合酶(iNOS)和Lamin B1的抗体来自Abcam(英国剑桥),af488标记的山羊抗兔IgG也来自Abcam。抗nrf2和二级山羊抗兔免疫球蛋白G (IgG)来自Bioworld (OH, USA),抗环氧化酶-2 (COX-2)来自Cell Signaling Technology (MA, USA)。重组IL-...
上海玉博生物科技有限公司 主要致力于“EMC microcollections ENB 试剂Enterbactin (iron-free) ”的生产销售。多年的“EMC microcollections ENB 试剂Enterbactin (iron-free) ”生产与销售的经验,与各行业新老用户建立了稳定的合作关系,我公司经营的产品名称深受广大用
发状分裂相关增强子1(hairy and enhancer of split homolog-1, Hes1)、E-钙黏蛋白(E-cadherin)单抗、波形蛋白(vimentin)单抗(美国Cell Signaling Technology公司),HRP标记的山羊抗兔二抗(美国Abcam公司),TE2000-U倒置生物显微镜(日本Ni...
Antibodies: CD81 and GM130 (Santa Cruz Biotechnology), SR-B1 (Abcam), β-Actin (Cell Signaling Technology) Lipid raft labeling A375 lipid rafts were labeled using cholera toxin subunit b (CTx-B) conjugates with Alexafluor 488 or Alexafluor 647 to (Life Technologies) at a final ...
(1:800)、Hes1(1:800)、E-cadherin(1:1 000)、vimentin(1:1 000)、β-actin(1:500),4 ℃摇床孵育过夜,TBST溶液洗膜3次×10 min,加入HRP标记的二抗(1:5 000),室温孵育 2 h,TBST溶液洗膜3次×10 min,加入ECL化学发光液,显影、成像,凝胶成像系统分析结果,目的蛋白相对表达量以其与内参蛋白条带灰度...
·n一,离心5min,4C。尽弃上清,加入 100mL细胞 laboratories,Inc), PKB(兔抗 鼠)(CellSignaling 裂解液B,吹打均匀。4C放置45min。再次转移入 1.5 Transmitter),Ser473磷酸化的PKB多克隆抗体(兔抗鼠) mL的Eppendorf管中,12000rm·in一,离心 10min, (CellSignalingTransmitter),~actin抗体(羊抗)(SantaCruz 4℃...
Our recent studies reveal an additional function of the actin cytoskeleton in the downregulation of BCR signaling, consequently contributing to the generation and maintenance of B-cell self-tolerance. In this review, we discuss how actin remodels its organization and dynamics in clos...
The antibody for β-actin (1:1000 dilution; Cell Signaling) was used as the endogenous control. The gels were run under the same experimental conditions and the original whole gel blots were included in the supplementary data. The levels of TNF-α, Apaf-1 and Casp7 protein in the serum ...
The data were analysed by Image J software and expressed as fold-change relative to the control group after normalizing against β-actin. Cell culture Five-day-old neonatal pig (Landrace, SCAU, Guangzhou, China) livers were perfused, and hepatocytes were isolated and purified using a two-step ...