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BMMC's and stromal cells were completely trypsinized with a 1:50 dilution of 20 mg % trypsin (Gibco/BRL, Burlington, Ontario, Canada) for 10 min. Cells were washed once by centrifugation for 8 min at 800 g followed by resuspension in complete media with serum. This wash was then ...
Immunopanning for final purification was carried out with mouse anti- rat -Thy1.1 antibody (Serotec, Oxford, UK) followed by 500 μl of baby rabbit complement (Cedarlane, Burlington, ON, Canada). Cell suspension was seeded on 60 mm Petri dishes or multiwells, maintained in DMEM, 10% FCS,...
Phone ready visualization: the interface was designed considering the layout of smart devices so that the researchers can easily explore, manage and follow the experiment from this kind of device. Figure 8. Real-time visualization on the web interface for seven days culturing N2A cells. (a) amp...
Flexcell© provides solutions for life science research. Flexcell® designs, develops and manufactures dynamic cell stretching culture systems and disposables.