leading to capillary dilation and intraglomerular microaneurysms after disease induction [15]. These changes contribute to secondary podocyte damage via altered physical forces and/or potential biochemical alterations of the glomerular basement membrane [16,17], which may promote proteinuria and ultimately ...
been demonstrated to sense impedance changes due to cell number increment, to validate the impedance-based system for cell culture monitoring as a result of cell attachment, and to determine the resistive properties of cell-electrode systems at low frequencies, mainly due to cell membrane capacity....
Samples were separated on a 10% polyacrylamide gel, the proteins were transferred onto a polyvinylidine fluoride membrane and blocked with blocking buffer (5% (w/v), defatted milk powder in Tris Buffered Saline with 0.1% (v/v) Tween20). Next, membranes were overnight incubated at 4 °C ...
Cells were recovered, lysed, and used for each array’s membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample. 2.9. Gene Silencing Assays Indicated cell lines were transfected in Opti-MEM medium ...
100 μL of the reagent was added to the 1 × 106 cells followed by homogenization and sonication (20 s) for membrane disruption. Cells were centrifuged for 5 min at 10,000× g to remove cell debris. Protein concentration was determined using a bicinchoninic acid (BCA) assay (cat. #2...
The method involves transferring the extracted RNA to a blotting membrane after first separating it by size using electrophoresis. Following the separation of the RNAs, the membrane is fixed, and sequences of interest are labeled by combining complementary DNA probes. Finally, hybridization with ...
Immunogenic cell death (ICD) in cancer represents a functionally unique therapeutic response that can induce tumor-targeting immune responses. ICD is characterized by the exposure and release of numerous damage-associated molecular patterns (DAMPs), whic
2.1. Polymer Electrolyte Membrane Fuel Cell System The PEMFC system generates energy using chemical reactions without harmful gases emission. For an FC system using hydrogen (anode) and oxygen (cathode) as reactants, the only reaction product is the drinkable water. The PEMFC is the best option...
Cell culture and cell scaffold engineering have previously developed in two directions. First can be ‘static into dynamic’, with proven effects that dynamic cultures have benefits over static ones. Researches in this direction have used several mechani
In general, according to ADME predictions, the synthesized chalcones A and B are the best candidates and could have the required cell membrane permeability and bioavailability without toxicity, followed by C and G. 3. Material and Methods 3.1. Calculation of the Molecular Descriptors The structures...