Incorporating multiple techniques into single-cell microfluidic devices to actively manipulate bioparticles has gained widespread interest in improving the capability of such devices. For instance, specifically labeling cells with immunomagnetic beads and subjecting the cells to a magnetic force via micromagn...
The Kir6.2 subunits form the potassium channel itself, whereas the SUR1 membrane proteins surround and influence the pore. Once the KATP channel is closed, the membrane potential is depolarized, action potential triggered, and L-type voltage-dependent Ca2+ channels are opened (Figure 2, step 5b...
CEACAM1 preferentially associates with these regions based on its oligomeric state, as shown by the solid and dashed orange arrows between the two membrane regions that indicate transport. The end state of the activated T cell consists of the clustering of CEACAM1 monomers within lipid rafts. B ...
Annexins are cytosolic proteins with conserved three-dimensional structures that bind acidic phospholipids in cellular membranes at elevated Ca2+levels. Through this they act as Ca2+-regulated membrane binding modules that organize membrane lipids, facilitating cellular membrane transport but also displaying ...
, horizontal cells near the outer plexiform layer, amacrine cells near the inner plexiform layer and Muller cell nuclei, which extend processes to the vitreal interface where they form the inner limiting membrane and toward the outer segments of the photoreceptor to the outer limiting membrane. ...
Furthermore, essential substances for functional tissue regeneration, such as key membrane proteins (e.g., ion channels and growth factor receptors) and intercellular and ECM connections, are preserved and remain intact due to the use of dispase. The preparation of cell sheets requires a delicate ...
circular and longitudinal smooth muscle layers; and serosa, the outermost layer enveloping the gut tube (Fig.1c). Therefore, the bottom of the crypt where ISCs reside is surrounded by a complex microenvironment composed of diverse types of cells and membrane and capillary structures (Powell et ...
(AT1) and downstream MAPKs. In addition to ACE2, other membrane receptors have been shown to interact with SARS-CoV-2 and trigger MAPK-mediated signalling; for example, spike protein activates p38 and ERK1/2 via the receptor CD147 in vivo [241] and in primary human cardiac pericytes [...
Membrane proteins (MPs), especially plasma MPs, play critical roles in cellular communication and interactions with their surroundings. They fulfill numerous functions, such as signal transduction, nutrient transport, cell adhesion, antigen presentation, drug extrusion, and many other enzymatic activities ...
The skeletal muscle T-tubule is a specialized membrane domain essential for coordinated muscle contraction. However, in the absence of genetically tractable systems the mechanisms involved in T-tubule formation are unknown. Here, we use the optically tra