CEACAM1 preferentially associates with these regions based on its oligomeric state, as shown by the solid and dashed orange arrows between the two membrane regions that indicate transport. The end state of the activated T cell consists of the clustering of CEACAM1 monomers within lipid rafts. B ...
The skeletal muscle T-tubule is a specialized membrane domain essential for coordinated muscle contraction. However, in the absence of genetically tractable systems the mechanisms involved in T-tubule formation are unknown. Here, we use the optically tra
Briefly, 1 × 104 TAMs were sorted and seeded onto Transwell polycarbonate filters (0.4 µm pore, 6.5 mm membrane diameter; Corning Incorporated, Corning, NY, USA). Simultaneously, 5 × 105 primary tumor cells were seeded into the lower compartments of Transwell chambers, allowing co...
Region-specific glycan maps were obtained with ∼120 N-glycan compositions in each region, revealing significant differences in “brain-type” glycans involving high mannose, bisecting, and core and antenna fucosylated species. Additionally, the cell membrane N-glycome of three functional regions of...
As photoelectrons for Chlorella cell-derived hydrogen production originate mainly from PSII, which captures solar energy and drives electron transfer in the thylakoid membrane (Fig. 2a), we determined the maximum potential quantum efficiency of PSII in the native and coated algal cells to evaluate th...
As single molecules traverse the cell membrane, which is a curved surface, the motion of divisome proteins like FtsW and FtsI may be underestimated. To address this curvature effect, we calibrated the diameter of the cell (or the septal ring) using the bright-field image.1 In 2D tracking,...
circular and longitudinal smooth muscle layers; and serosa, the outermost layer enveloping the gut tube (Fig.1c). Therefore, the bottom of the crypt where ISCs reside is surrounded by a complex microenvironment composed of diverse types of cells and membrane and capillary structures (Powell et ...
and it is also thought to be important for the infection of highly pathogenic viruses in which the integrity of cholesterol transport is vital. When the EBOV glycoprotein (GP) binds with NPC1 at the endosomal membrane, the viral material is released into the host cell, starting the infective...
GO terms specifically enriched in pleiotropic genes based on our definition are dominated by terms that relate to multicellularity, such as membrane signaling, cell-to-cell communication and cell migration (Supplementary Fig. 7). For pleiotropy that is specifically found with CRISPR screens, we find...
3C and D). The specific gene marker set for cardiomyocyte-0 was enriched in pathways related to fatty acid metabolism, fatty acid oxidation, and lipid oxidation, whereas the gene set for cardiomyocyte-2 was enriched in pathways related to myocardial action potentials, membrane potential regulation,...