The LC3 immunofluorescent staining in both cytoplasm and nucleus slightly increased after ATO treatment in NCI-H1975 cells (Fig. 2d). This increase became more significant when NH4Cl or bafilomycin A1 (BafA1) was added to block the protein degradation by proteases in lysosomes and in ...
1a). The localization of PLAC8 in LC cells (H1299 and H322) was confirmed using immunofluorescence staining, and PLAC8 was localized in both the nucleus and cytosol of LC cancer cells (Fig. 1b and Fig. S2). Additionally, correlations between PLAC8 expression and the clinical outcomes of...
The nuclear matrix is mainly composed of a protein framework (nuclear lamina), which is key to the organization of chromatin and nuclear function. The lamins are type V intermediate filaments (similar to those found in the cytoplasm) that have been specifically targeted to the nucleus. The polym...
7a). Overall, our results suggested that scCM exhibits an important angiogenic activity while lacking the negative effects of VEGF-A on vascular barrier properties. To the best of our knowledge, this is the first study evaluating the effect of EPC-secretome produced under high-scale conditions in...
1-32B). Their axons form the nerve fibre layer on the innermost surface of the retina and synapse with cells in the lateral geniculate nucleus of the thalamus. The axons form bundles separated and ensheathed by glial cells (Fig. 1-34E). The bundles leave the eye to form the optic nerve...
cytoplasm and impede their reentry into the nucleus, thereby inhibiting FOXO transcriptional activity. In most cases, the phosphorylation modification of FOXO drives its localization to the cytoplasm, but a few phosphorylated forms promote FOXO entry into the nucleus. Under oxidative stress, ...
Cell type-dependent impact of hypoxic conditions on CD133 expression.aUnder hypoxic conditions and in certain types of cancer as indicated, HIF-1α and HIF-1β are translocated into the nucleus, where they form a heterodimeric transcription factor that binds to hypoxia-responsive elements (HRE),...
the ADAR p150 isoform serves as the nuclear export signal (NES) and Z-DNA binding domain. Although both ADAR1 p110 and ADAR1 p150 can move between the nucleus and cytoplasm, p110 is predominantly located in the nucleus, while p150 is primarily present in the cytoplasm [9]. The functional...
nucleus in response to TNF. In some cells, this is a single cycle of nuclear translocation. In other cells, delayed negative feedback loops in the NF-κB system, most importantly via IκBα and A20 [18,19,20,21,22], drive regular cycles of NF-κB translocation into and out of the ...
LINC01116 content in both cell cytoplasm and cell nucleus were examined by RT-qPCR and AGE (agarose gel electrophoresis) analyses. Fluorescence in situ hybridization The fixed J82 and T24 cells were rinsed in PBS, dehydrated, and air-dried for cultivating in hybridization buffer with LINC01116-...