The LC3 immunofluorescent staining in both cytoplasm and nucleus slightly increased after ATO treatment in NCI-H1975 cells (Fig. 2d). This increase became more significant when NH4Cl or bafilomycin A1 (BafA1) wa
The nuclear matrix is mainly composed of a protein framework (nuclear lamina), which is key to the organization of chromatin and nuclear function. The lamins are type V intermediate filaments (similar to those found in the cytoplasm) that have been specifically targeted to the nucleus. The polym...
White blood cells are subdivided into five parts, according to the nucleus and cytoplasm each part is differentiating from the other as shown in Fig. 3 [68]. So WBC nucleus segmentation is a basic requirement that is mostly done by color space conversion and the k-means clustering algorithm ...
1a). The localization of PLAC8 in LC cells (H1299 and H322) was confirmed using immunofluorescence staining, and PLAC8 was localized in both the nucleus and cytosol of LC cancer cells (Fig. 1b and Fig. S2). Additionally, correlations between PLAC8 expression and the clinical outcomes of...
differentiation process would have allowed the separation of the nucleus from the rest of the cell. Hardened membranes around the nucleus and the cell emerged as the result of the contact between two “phases”, i.e. the nucleus/cytoplasm or cytoplasm/environment, respectively. Although Schleiden ...
1i). Of the three NF-κB subunits including RELA, RELB and c-Rel, all 3 patients expressed at least two subunits of NF-κB in both cytoplasm and nucleus (patient 3 was negative for c-Rel) (Fig. 1j-l), indicating the nuclear translocation and activation of the NF-κB pathway. Table...
Caspases are synthesized as inactive proenzymes, which upon activation cleave various substrates in the cytoplasm or nucleus. This leads to many of the morphologic features of apoptotic cell death, for example, polynucleosomal DNA fragmentation, loss of overall cell shape, and nuclear shrinking [22,...
Zn2+ is required for the activity of many mitochondrial proteins, which regulate mitochondrial dynamics, apoptosis and mitophagy. However, it is not understood how the proper mitochondrial Zn2+ level is achieved to maintain mitochondrial homeostasis. Using Caenorhabditis elegans, we reveal here that a...
We found that, in the presence of bristles lacking the socket cell, surrounding epidermal cells can still upregulate the expression of Diap1-GFP and express neur > RFP, albeit in an aberrant position (Extended Data Fig. 4c). Conversely, however, in the presence of bristles lacking the ...
LINC01116 content in both cell cytoplasm and cell nucleus were examined by RT-qPCR and AGE (agarose gel electrophoresis) analyses. Fluorescence in situ hybridization The fixed J82 and T24 cells were rinsed in PBS, dehydrated, and air-dried for cultivating in hybridization buffer with LINC01116-...