Cell-free gene expression (CFE) emerged as an alternative approach to living cells for specific applications in protein synthesis and labelling for structural biology and proteomics studies. CFE has since been repurposed as a versatile technology for synthetic biology and bioengineering. However, taking...
Cell-free biology is the activation of biological processes without the use of intact living cells. It has been used for more than 50 years across the life sciences as a foundational research tool, but a recent technical renaissance has facilitated high-
Cell-free expression (CFE) systems have been used extensively in systems and synthetic biology as a promising platform for manufacturing proteins and chemicals. Currently, the most widely used CFE system is in vitro protein transcription and translation platform. As the rapidly increased applications ...
Here, we introduce a programmable vector - circular single-stranded DNA (CssDNA), which is shown to be processed by DNA and RNA polymerases for gene expression in a yeast-based CFE system. CssDNA is already widely employed in DNA nanotechnology due to its addressability and programmability. To...
(Zheng et al., 2017). The majority of the EGFP+Club cells were CD34−, but SCA-1+and integrin α6+, and the CFE was approximately 10%, greater than the possible ATII cell contamination (Zheng et al., 2017). The phenomenon of bronchiolarization, where injury-resistant airway cells ...
Expressway Maxi Cell-Free E. coli Expression System with pEXP5-NT/TOPO and pEXP5-CT/TOPO vectors Introduction to in vitro protein expression In vitro protein expression (also known as in vitro translation, cell-free protein ex...
system an OD600of 40 was used, which is equivalent to 3 μM CYP154C5 and an ETC activity of 9 U/mL (corresponding to a specific activity of 1.4 U/mg) as determined by analysis of cell-free extract that was obtained after cell disruption. In the case of bioconversions using CFE 3 ...
The response can, for example, be measured by determining the signal transduction enhanced or inhibited by the composition via a reporter system, by measuring downstream effects such as gene expression, or by measuring the physiological effects of the composition. Likewise, the effects of antisense/...
Cell-free gene expression (CFE) systems from crude cellular extracts have attracted much attention for biomanufacturing and synthetic biology. However, activating membrane-dependent functionality of cell-derived vesicles in bacterial CFE systems has been
Spaceflight induces an immune response in astronauts. To better characterize this effect, we generated single-cell, multi-ome, cell-free RNA (cfRNA), biochemical, and hematology data for the SpaceX Inspiration4 (I4) mission crew. We found that 18 cytokin