Cell-free biology is the activation of biological processes without the use of intact living cells. It has been used for more than 50 years across the life sciences as a foundational research tool, but a recent technical renaissance has facilitated high-
Cell-free gene expression (CFE) emerged as an alternative approach to living cells for specific applications in protein synthesis and labelling for structural biology and proteomics studies. CFE has since been repurposed as a versatile technology for synthetic biology and bioengineering. However, taking...
A cell-free system for preparative gene expression is described. It is composed of DNA-free Escherichia coli extract and added plasmid DNA; coupled transcription-translation proceeds with a continuous flow of the feeding solution containing nucleoside triphosphates and amino acids. The system works at...
细胞游离DNA(cell-free DNA,cfDNA)是从细胞释放到体循环中的DNA片段,cfDNA存在于各种体液中,随组织损伤、癌症和炎症反应等发生浓度变化。生理情况下,血液中的cfDNA主要来源于人体白细胞的坏死和凋亡,而在某些疾病及特殊状态下,其他细胞也会释放游离DNA进入血液中,包括源于肿瘤细胞的循环肿瘤DNA。 图源:文献 由于cfDNA...
2022年3月31日,来自斯坦福大学的Maximilian Diehn和Ash A. Alizadeh研究组在Nature Biotechnology上发表题为Inferring gene expression from cell-free DNA fragmentation profiles的文章,提出了提高精确度和灵敏度的新方法。 首先,作者假设,相较于受静默启动子调控,受高度活化的启动子调控的游离DNA片段其切割模式要更为随...
相关研究结果发表在2024年2月15日的Cell期刊上,论文标题为“A retroviral link to vertebrate myelination through retrotransposon-RNA-mediated control of myelin gene expression”。 图片来自Cell, 2024, doi:10.1016/j.cell.2024.01.011 这些作者考察了髓鞘(myelin)的起源,其中髓鞘是一种脂肪组织绝缘层,形成于神经...
Gene transfer to human cells: transducing phage lambda plac gene expression in GMI-gangliosidosis fibroblasts. Genetic information from the bacterium Escherichia coli was transferred to human cells by means of the specialized transducing phage lambda plac carrying t... Horst,Kluge,Beyreuther,... - 《...
在第一篇文章“Toehold Switches: De-Novo-Designed Regulators of Gene Expression”中,尹鹏等人就报道了一种从头设计(de-novo-designed)的原核细胞调控因子:riboregulators,研究人员将其称为支点开关(toehold switches,生物通译)。 这种开关能对任何序列的同源RNAs作出应答,激活基因表达,从而提供了一种高水平正交性,...
In brief, the hypocotyl was chopped into small discs with scalpel blade and immersed in 3–5 ml RNase-free enzyme solution (1.5% cellulase R10, 1.5% macerozyme R10, 0.4M mannitol, 10mM KCl, 10mM CaCl2, and 0.1% BSA). Then digested for 4 h with gentle shaking (30 r/min) in the ...
Cell-free gene expression (CFE) emerged as an alternative approach to living cells for specific applications in protein synthesis and labelling for structural biology and proteomics studies. CFE has since been repurposed as a versatile technology for synthetic biology and bioengineering. However, taking...