Cell-free gene expression (CFE) emerged as an alternative approach to living cells for specific applications in protein synthesis and labelling for structural biology and proteomics studies. CFE has since been repurposed as a versatile technology for synthetic biology and bioengineering. However, taking...
细胞游离DNA(cell-free DNA,cfDNA)是从细胞释放到体循环中的DNA片段,cfDNA存在于各种体液中,随组织损伤、癌症和炎症反应等发生浓度变化。生理情况下,血液中的cfDNA主要来源于人体白细胞的坏死和凋亡,而在某些疾病及特殊状态下,其他细胞也会释放游离DNA进入血液中,包括源于肿瘤细胞的循环肿瘤DNA。 图源:文献 由于cfDNA...
Cell-free gene expression (CFE) emerged as an alternative approach to living cells for specific applications in protein synthesis and labelling for structural biology and proteomics studies. CFE has since been repurposed as a versatile technology for synthetic biology and bioengineering. However, taking...
2022年3月31日,来自斯坦福大学的Maximilian Diehn和Ash A. Alizadeh研究组在Nature Biotechnology上发表题为Inferring gene expression from cell-free DNA fragmentation profiles的文章,提出了提高精确度和灵敏度的新方法。 首先,作者假设,相较于受静默启动子调控,受高度活化的启动子调控的游离DNA片段其切割模式要更为随...
A Cell-Free Gene Expression Platform for Discovering and Characterizing Stop Codon Suppressing tRNAs 来自 ACS 喜欢 0 阅读量: 17 作者:K Seki,JL Galindo,AS Karim,MC Jewett 摘要: Non-canonical amino acids (ncAAs)can be incorporatedinto peptidesand proteins to create new properties and functions. ...
A cell-free system for preparative gene expression is described. It is composed of DNA-free Escherichia coli extract and added plasmid DNA; coupled transcription-translation proceeds with a continuous flow of the feeding solution containing nucleoside triphosphates and amino acids. The system works at...
作者采用了不含微生物的线虫野生株系N2 (microbe-free, N2 strain),分别用两种枯草芽孢杆菌饲喂线虫:一种为野生型,另一种是一氧化氮合酶突变的菌株(Δnos),该突变菌株不能有效的合成一氧化氮。待线虫生长到幼虫的第 4 阶段(Larval stage 4, L4)时,作者提取了线虫体内的总蛋白,并且特异的分离了巯基亚硝基化修...
Expressway Maxi Cell-Free E. coli Expression System with pEXP5-NT/TOPO and pEXP5-CT/TOPO vectors Introduction to in vitro protein expression In vitroprotein expression (also known asin vitrotranslation, cell-free protein expression...
2019年4月18日,美国马萨诸塞州总医院的学者在Cell上发文Protein Sequence Editing of SKN-1A/Nrf1 by Peptide:N-Glycanase Controls Proteasome Gene Expression,以线虫为研究对象,建立了PNG-1 PNGase和DDI-1天冬氨酸蛋白酶激活SKN-1A的分子机制。本文发现SKN-1A的功能化需要糖基化、去糖基化和蛋白水解作用,并在...
Cell-free gene expression: an expanded repertoire of applications 2020, Nature Reviews Genetics Anti-CRISPR-mediated control of gene editing and synthetic circuits in eukaryotic cells 2019, Nature Communications Widespread anti-CRISPR proteins in virulent bacteriophages inhibit a range of Cas9 proteins 2018...