Cell-free gene expression (CFE) emerged as an alternative approach to living cells for specific applications in protein synthesis and labelling for structural biology and proteomics studies. CFE has since been repurposed as a versatile technology for synthetic biology and bioengineering. However, taking...
Cell-free biology is the activation of biological processes without the use of intact living cells. It has been used for more than 50 years across the life sciences as a foundational research tool, but a recent technical renaissance has facilitated high-
Cell-free gene expression (CFE) emerged as an alternative approach to living cells for specific applications in protein synthesis and labelling for structural biology and proteomics studies. CFE has since been repurposed as a versatile technology for synthetic biology and bioengineering. However, taking...
but a recent technical renaissance has facilitated high-yielding (grams of protein per litre), cell-free gene expression systems from model bacteria, the development of cell-free platforms
Baranov et al., “Gene expression in a cell-free system on the preparative scale”, Gene, 84(1989) 463-466.Baranov VI, Morozov IYu, Ortlepp SA, Spirin AS. Gene expression in a cell-free system on the preparative scale. Gene. 1989 Dec 14; 84 (2):463–466....
细胞游离DNA(cell-free DNA,cfDNA)是从细胞释放到体循环中的DNA片段, cfDNA存在于各种体液中,随组织损伤、癌症和炎症反应等发生浓度变化。生理情况下,血液中的cfDNA主要来源于人体白细胞的坏死和凋亡,而在…
相关研究结果发表在2024年2月15日的Cell期刊上,论文标题为“A retroviral link to vertebrate myelination through retrotransposon-RNA-mediated control of myelin gene expression”。 图片来自Cell, 2024, doi:10.1016/j.cell.2024.01.011 这些作者考察了髓鞘(myelin)的起源,其中髓鞘是一种脂肪组织绝缘层,形成于神经...
Cell-free systems Calls for Papers Immobilization of Microbial Cells for Industrial and Environmental Applications Edited by: Pankaj Bhatt, Zhen Li, and Priscilla Amaral Engineering Microbiomes for Green Technologies Edited by: Vijai Kumar Gupta, Jack A. Gilbert, and Meisam Tabatabaei ...
在第一篇文章“Toehold Switches: De-Novo-Designed Regulators of Gene Expression”中,尹鹏等人就报道了一种从头设计(de-novo-designed)的原核细胞调控因子:riboregulators,研究人员将其称为支点开关(toehold switches,生物通译)。 这种开关能对任何序列的同源RNAs作出应答,激活基因表达,从而提供了一种高水平正交性,...
另外,2022年3月14日,原中国科学院分子植物科学卓越创新中心/中国科学院上海植物逆境生物学研究中心朱健康团队(现单位南方科技大学,何力为第一作者)在Nature Communications 在线发表题为“DNA methylation-free Arabidopsis reveals crucial roles of DNA methylation in regulating gene expression and development”的研究论文...