Cell-free biology is the activation of biological processes without the use of intact living cells. It has been used for more than 50 years across the life sciences as a foundational research tool, but a recent technical renaissance has facilitated high-
Cell-free gene expression (CFE) emerged as an alternative approach to living cells for specific applications in protein synthesis and labelling for structural biology and proteomics studies. CFE has since been repurposed as a versatile technology for synthetic biology and bioengineering. However, taking...
In [54], the exact closed-form expression for the achievable SE of a dubbed, enhanced, normalized conjugate beamforming system was derived. Besides, they developed an optimal max–min fairness power control algorithm that is only dependent on LSF quantities. Different iterative matrix inversion ...
Production of bispecific antibodies in “knobs-into-holes” using a cell-free expression system Mabs, 7 (2015), pp. 231-242 CrossrefView in ScopusGoogle Scholar 27 Y. Lu, J.P. Welsh, J.R. Swartz Production and stabilization of the trimeric influenza hemagglutinin stem domain for potentially...
Central Nervous System PET-CT Imaging Reveals Regional Impairments in Pediatric Patients with Wolfram Syndrome.Zmyslowska A,et al.PLoS One.2014 Dec 26. --- Gene expression changes in serotonin, GABA-A receptors, neuropeptides and ion channels in the dorsal raphe nucleus of adolescent alcohol-...
Co-expression network was constructed using WGCNA. Module- and hub-based methods were adopted to predict the functions of lncRNAs. Results A total of 77,382 cells during the differentiation process of hESCs toward pancreatic progenitors were sequenced. According to the single-cell map, the cells ...
The proposed method TOAST is essentially a feature selection method and thus could be used with existing reference-free algorithms to improve RF deconvolution. In order to evaluate the utility and flexibility of TOAST, we conduct extensive simulation and real data analyses of both gene expression dat...
Adipose-derived stem cells (ADSCs) have become one of the most utilized adult stem cells due to their abundance and accessibility. Recent studies have shown that paracrine cytokines, exosomes, and other active substances are the main factors through whic
[1] Restoration of FMRP expression in adult V1 neurons rescues visual deficits in a mouse model of fragile X syndrome 2022,《Protein & Cell》,IF:14.870 DOI:10.1007/s13238-021-00878-z [2] Astrocytic α4nAchRs Signaling in the Hippocampus Governs the Formation of Temporal Association Memory ...
(Methods). We used two different approaches to add varying concentrations of protein ligands to the cell-free expression system: (i) co-expression or (ii) co-addition. For co-expression, we added varying amounts of a second expression plasmid to the cell-free expression system to ...