细胞周期与细胞凋亡检测试剂盒(Cell Cycle and Apoptosis Analysis Kit)是一种采用经典的碘化丙啶染色方法进行细胞周期与细胞凋亡分析。 碘化丙啶(Propidium Iodide,简称PI)是一种双链DNA的荧光染料。碘化丙啶和双链DNA结合后可以产生荧光,并且荧光强度和双链DNA的含量成正比。细胞内的DNA被碘化丙啶染色后,可以用...
细胞周期与细胞凋亡检测试剂盒(Cell Cycle and Apoptosis Analysis Kit)采用了经典的碘化丙啶染色(Propidium staining,即PI staining)方法对细胞周期与细胞凋亡进行分析。 碘化丙啶 (Propidium,PI) 是一种双链DNA荧光染料,其嵌入双链DNA后可以产生荧光,并且荧光强度和双链DNA的含量成正比。细胞内的DNA被PI染色后,可...
1、细胞周期与凋亡检测试剂盒(PI法)(Cell Cycle and Apoptosis Analysis Kit (PI)载体酶切:将表达质粒用限制性内切酶(同引物的酶切位点)进行双酶切,酶切产物行琼脂糖电泳后,用胶回收Kit或冻融法回收载体大片段。 2、PCR产物双酶切后回收,在T4DNA连接酶作用下连接入载体。
1995 . Cell cycle and apoptosis: common pathways to life and death . J Cell Biochem 58 : 175 – 180 . TODO: clickthrough URL MedlineKing KL, Cidlowski JA (1995) Cell cycle and apoptosis: common pathways to life and death. J Cell Biochem 58(2):175–180...
细胞周期与细胞凋亡检测试剂盒(Cell Cycle and Apoptosis Analysis Kit)是一款采用经典的碘化丙啶染色法进行细胞周期和细胞凋亡分析的试剂盒,通过流式细胞仪进行分析。本试剂盒通常用于贴壁细胞或悬浮细胞的细胞周期与凋亡检测。对于组织样品,需经消化成单细胞后才可用此试剂盒进行检测。
Apoptosis and the cell cycle. Curr Opin Cell Biol 1995;7:825-834.Evan GI, Brown L, Whyte M, Harrington E. Apop- tosis and the cell cycle. Curr Op Cell Bio 1995; 7: 825- 834.Meikrantz W, Schlegel R. Apoptosis and the cell cycle. Journal of Cellular Biochemistry. 1995; 58 :160...
An argument in favor of a link between the cell cycle and apoptosis arises from the accumulated evidence that manipulation of the cell cycle may either prevent or induce an apoptotic response. This linkage has been recognized for tumor suppressor genes such as p53 and RB, the dominant oncogene,...
细胞周期与细胞凋亡检测试剂盒(Cell Cycle and Apoptosis Analysis Kit)是一款采用经典的碘化丙啶染色法进行细胞周期和细胞凋亡分析的试剂盒,通过流式细胞仪进行分析。本试剂盒通常用于贴壁细胞或悬浮细胞的细胞周期与凋亡检测。对于组织样品,需经消化成单细胞后才可用此试剂盒进行检测。 碘化丙啶(Propidium ...
Beneath the complexity and idiopathy of every cancer lies a limited number of 'mission critical' events that have propelled the tumour cell and its progeny into uncontrolled expansion and invasion. One of these is deregulated cell proliferation, which, t
Some cell cycle regulators can influence both cell division and programmed cell death. The linkage of cell cycle and apoptosis has been recognized for c-Myc, p53, pRb, Ras, PKA, PKC, Bcl-2, NF-kappa B, CDK, cyclins and CKI. This review summarizes the different functions of the proteins...