细胞周期检测试剂盒(Cell Cycle and Apoptosis Kit)是一种采用经典的碘化丙啶(Propidium Iodide ,即PI)染色方法进行细胞周期与细胞凋亡分析的检测试剂盒。 碘化丙啶是一种双链DNA的荧光染料。碘化丙啶和双链DNA结合后可以产生荧光,并且荧光强度和双链DNA的含量成正比。细胞内的DNA被碘化丙啶染色后,可以用流式细...
细胞周期与细胞凋亡检测试剂盒(Cell Cycle and Apoptosis Analysis Kit)采用了经典的碘化丙啶染色(Propidium staining,即PI staining)方法对细胞周期与细胞凋亡进行分析。 碘化丙啶 (Propidium,PI) 是一种双链DNA荧光染料,其嵌入双链DNA后可以产生荧光,并且荧光强度和双链DNA的含量成正比。细胞内的DNA被PI染色后,可...
some degree: many oncogenes that promote cell cycle progression also induce apoptosis; damage to the cell cycle or to DNA integrity is a potent trigger of apoptosis; and the key tumour suppressor proteins, p105rb and p53, exert direct effects both on cell viability and on cell cycle ...
细胞周期检测试剂盒(Cell Cycle and Apoptosis Analysis Kit)是一种采用经典的碘化丙啶染色(Propidium staining,即 PI staining)方法进行细胞周期与细胞凋亡分析的检测试剂盒。
细胞周期与细胞凋亡检测试剂盒(Cell Cycle and Apoptosis Analysis Kit)是一款采用经典的碘化丙啶染色法进行细胞周期和细胞凋亡分析的试剂盒,通过流式细胞仪进行分析。本试剂盒通常用于贴壁细胞或悬浮细胞的细胞周期与凋亡检测。对于组织样品,需经消化成单细胞后才可用此试剂盒进行检测。
Meikrantz W and Schlegel R (1995) Apoptosis and the cell cycle. J. Cell. Biochem. 58: 160-174Evan GI, Brown L, Whyte M, et al. Apoptosis and the cell cycle. Curr Opin Cell Biol 1995;7:825-34.Meikrantz W, Schlegel R. Apoptosis and the cell-cycle. J Cell Biochem. 1995;58:160...
Beneath the complexity and idiopathy of every cancer lies a limited number of 'mission critical' events that have propelled the tumour cell and its progeny into uncontrolled expansion and invasion. One of these is deregulated cell proliferation, which, t
1、细胞周期与凋亡检测试剂盒(PI法)(Cell Cycle and Apoptosis Analysis Kit (PI)载体酶切:将表达质粒用限制性内切酶(同引物的酶切位点)进行双酶切,酶切产物行琼脂糖电泳后,用胶回收Kit或冻融法回收载体大片段。 2、PCR产物双酶切后回收,在T4DNA连接酶作用下连接入载体。
Some cell cycle regulators can influence both cell division and programmed cell death. The linkage of cell cycle and apoptosis has been recognized for c-Myc, p53, pRb, Ras, PKA, PKC, Bcl-2, NF-kappa B, CDK, cyclins and CKI. This review summarizes the different functions of the proteins...
5) cell cycle and apoptosis-regulatory protein-1 促凋亡基因细胞周期和凋亡调控蛋白1 1. Objective To explore the effects of oxidative stress on the expression of cell cycle and apoptosis-regulatory protein-1(CCAR1) in lung epithelial type Ⅱ cells. 目的探讨氧化应激对肺泡Ⅱ型上皮细胞中促凋亡...