Laboratory Centrifuge Brand Name Innova Model Number SIB4 Max. speed(rpm) 4500RPM Max RCF 2000xg Max. capacity 36(0.25~1ml)x12 Speed Accuracy ±30rpm Timer Range 1min~99min Noise ≤60dB(A) Voltage AC220V±22V,50Hz 10A or custom Power 100W Dimensions (W*D*H)(mm) 456x330x266 Weight...
The cells were transferred to a 1.5 mL centrifuge tube, harvested (500 g, 5 min, 4 °C) and resuspended in ice-cold Labelling Buffer (0.2 mL). The metabolic cell surface labelling and in-gel fluorescence analysis was subsequently performed as explained above. Protein expression was...
For PI staining and flow cytometric analysis, fixed cells were washed and centrifuge in 500 g, for 5 min. Cell pellet then was resuspended in 400 μl PBS supplemented with 8 μl RNAse (1 μg/ml) and 4 μl PI (2 μg/ml). Samples were incubated for 10 min in ...
available kits are mostly based on differential centrifugation in media of high viscosity, such as sucrose [21,28,30,31] and are designed to fractionate cells in a short period of time (less than 2 h), in an easy and efficient way, usually only requiring a bench top centrifuge [30,42...
Cells were centrifuged at 500 g for 5 min in a pre-chilled (4°C) fixed-angle centrifuge. After centrifugation, the supernatant was carefully aspirated not to perturb the cell pellet. Cell pellets were then resuspended in 35 μl of ATAC-lysis buffer (ATAC-RSB containing 0.1% NP40, 0.1%...
Laboratory Centrifuge Brand Name Cence Model Number CHT210R Max Speed 21000r/min Max Capacity 4x750ml Max RCF 32752xg Timing Range 1min -99h59min or 1s-99min59s Timing Mode start timing , speed timing and continuous timing. Power supply AC220V±22V 50Hz Dimension(W*D*H) 713x681x39...
to pellet the membrane and remove ribosomes, RNAs, and other cytosolic molecules. The pellet was resuspended in storage buffer (10 mM Tris-HCl pH 7.5, 1 mM EDTA, and 10 μL RNasin per 10 mL of solution). Centrifuge at 100,000×gfor 35 min. Membrane-associated RNA concent...
when compared either fixed angled centrifuge (InstraSpin, Process for PRF). In conclusion, the present study revealed a novel/accurate method to quantify cells following PRF protocols. Furthermore, PRF produced via horizontal centrifugation accumulated a higher number and concentration of platelets/...
Automated cellular sample preparation using a Centrifuge-on-a-Chip. Lab Chip 2011, 11, 2827–2834. [CrossRef] [PubMed] 42. Bow, H.; Pivkin, I.V.; Diez-Silva, M.; Goldfless, S.J.; Dao, M.; Niles, J.C.; Suresh, S.; Han, J. A microfabricated deformability-based flow ...
Briefly, cells were pelleted by 13,000 rpm for 1.5 min in a tabletop centrifuge, cells were resuspended in 50 μL Transposase Reaction Mix (25 μL 2x TD Buffer, 2.5 μL Transposase Enzyme from Illumina Nextera Cat# FC121-1030, 22 μL nuclease-free water, 0.5 μL ...