TECH TIP # 40 Convert between times gravity (×g) and centrifuge rotor speed (RPM) TR0040.1 Introduction Certain procedures necessitate precise centrifugation conditions, which must be specified in terms of relative centrifugal force (RCF) expressed in units of gravity (times gravity or × g). ...
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Two mL of fermentation sample was spun in a centrifuge (Eppendorf, UK) at 5000 rpm for 5 min to remove cell. The liquid suspension was then used as crude enzyme solution. A reaction mixture containing 0.9 mL of 0.05 mM citrate buffer (pH 5.0), 1.0 mL autoclaved starch solution (1%, w...