TruStain FcX block (BioLegend, 422301) was added before mixing and incubating for 10 min on ice. Next, 1 µg of TotalSeqA antibody was diluted in cell staining buffer, and 50 µl of this antibody dilution was added to the blocked cells before incubation on ice for 30 min....
25. Gabriel M, Balle D, Bigault S, Pornin C, Getin S, Perraut F, Block MR, Chetelain F, Picollet‐D’hahan N, Gidrol X, Haguet V. Time‐laps contact microscopy of cell cultures based on non‐coherent illumination. Scientific Reports. 2015; 5: 14532. doi:10.1038/srep14532 26....
A medication used in cancer treatment primarily by targeting the thymidylate synthase to block thymidine synthesis. Damage-associated molecular patterns A group of biomolecules that are enriched upon non-infectious inflammatory responses such as during the process of wound healing. Thioredoxin A class of...
A block alterable memory cell (400) has a select control gate (408; 524) extending from a floating gate region (410; 520) to a drain region (402; 528). The block alterable memory cell comprises a substrate (401; 502) that further includes a source implant region (406), a buried im...
Block GD, Locker J, Bowen WC, Peterson BE, Katyal S, Strom SC, Riley T, Howard TA, Michalopoulos GK (1996) Population expansion, clonal division, and specific differentiation patterns in primary cultures of hepatocytes by HGF/SF, EGF, and TGFα in a chemically defined (HGM) medium. J ...
Nuclear compartmentalization via phase separations. Schematics illustrating different types of phase separations implicated in the nuclear organization:aliquid-liquid phase separation of liquid components (liquid A,blue, and liquid B,pink),bmicrophase separation of chromatin fiber as a block-copolymer (mono...
This plasticity appears to depend on gene activity, since upon block of polyploidization in subperineurial glial cells, septate junctions are interrupted (Unhavaithaya and Orr-Weaver, 2012). The ability of subperineurial glial cells to react to changing barrier requirements can also be seen in ...
To ensure representativeness and homogeneity, the whole dots were observed in tissue microarray, and 5 fields of immune cell highly infiltrated tumor area were selected in the FFPE block slide. All the evaluation was performed by the same pathologist blinded to patient characteristics. Tumor ...
In HeLa-NC cells, γH2AX levels gradually increased 5 h after release from the G1 block, and reached a peak at 8-10 h, i.e. with the majority of cells in G2/M-phase. This cell cycle associated increase of γH2AX levels in the DNA-PKcs-depleted HeLa-H1 cells was much lower than...
(Fig.1gand Extended Data Fig.1a). This procedure partitions the data in such a way that the cell–cell similarity matrix has tight block structure along the diagonal; each partition is a group of cells that best represents a cell state and defines a metacell. The number of metacells is ...