Both assays revealed a substantial cell-to-cell heterogeneity. To interpret the results systematically, we also derived a stochastic mathematical model of the intracellular viral life cycle. This model allows us to simulate the extent to which molecular noise affects single-cell IAV infections and to...
process, where uptake ofmacromoleculesor nanomaterials might alter the vesicle buoyant density and therefore necessitate an adaptedpurificationprotocol for each nanoparticle[85]. Therefore it seems advisable to confirm findings by the cellular fractionation assay with other assays, as the ones described ...
Most importantly, we assessed the effect of KBTBD4WT, KBTBD4R313PRR and KBTBD4P311PP expression on stemness using two well established assays for cancer stem cells, neurosphere formation ability and ALDH activity [22, 23]. Previous work showed that medulloblastoma initiating cells isolated based...
However, treatment with any of the inhibitors alone or in combination did not change the avidity of CD8+ T cells in peptide titration assays with T2.A1 cells. T-helper cell/DC interaction is a bi-directional process that normally results in DC activation. Vemurafenib and vemurafenib/cobimetinib...
2.11. Cell–Cell Interaction Analysis The cell–cell interaction analysis was performed by CellPhoneDB based on receptor- ligand interactions between two cell types/subtypes [25]. Cluster labels of all cells were randomly permuted 1000 times to calculate the null distribution of average ligand–...
看文献发现一种细胞计数的方法叫ATP-based cell proliferation assays ，请问有人知道这是一种什么样的...
PPT1 was also demonstrated to form oligomers by size-exclusion chromatography and co-immunoprecipitation assays. Finally, the consequences of disease mutations were analyzed in the perspective of our new results, suggesting that the mutations increase both the degree of glycosylation of PPT1 and its ...
Based on the half-maximum growth inhibitory concentration (GI50) of dasatinib reported previously (18), 13 sensitive and 13 resistant NSCLC cell lines were preselected. For these 26 cell lines, we repeated viability assays to verify the reported GI50 values. We chose the median GI50 as classifica...
However, in the absence of Tax, mutation of the GRE significantly decreases basal LTR activity as shown in reporter-based assays [25]. This raises the possibility that this substitution may have allowed a better silencing of viral transcription in the lymphosarcoma strains, as a strategy to ...
This section lists the equipment and supplies common to most cell culture laboratories, as well as beneficial equipment that allows the work to be performed more efficiently or accurately, or permits wider range of assays and analyses. ? Note that this list is not all inclusive; the requirements...