Cell-BasedAssay(细胞活性、毒性和凋亡)
PromegaPromegaCellBasedAssayCellBasedAssaySherryZhouSherryZhouKeyAccountSpecialistKeyAccountSpecialistPromegaPromegaCorporationCorporationPromegaPromegaisakeyvendorofgreatCBAisakeyvendorofgreatCBACellViabilityCellViability、、CytotoxicityCytotoxicity&Apoptosis&ApoptosisCellViabilityCellViability为什么做细胞增殖检测••初步观察细...
Cell-based assays allow for the measurement and quantification of the potency and activity of biological reagents. Functional validation Cell-based activity assays provide a means to validate the functionality of biological reagents, assessing whether they are capable of interacting with specific cellular ...
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Reporter gene assays (RGAs) are Mechanism of Action (MOA)-related, less variable, higher sensitive and labor-saving. They have been increasingly adopted as quality control of biopharmaceuticals [29,30]. Herein, an RGA for determining the bioactivity of therapeutic rhKGF was developed based on ...
Along with academic and charitable organizations, transfusion centers have ventured into the stem cell field, with the aim of testing of novel cell-based therapeutics in a clinical setting for future marketing approval. The fact that quality management structures, which are required for compliance with...
These results were confirmed by co-immunoprecipitation assays (Fig. 2b), showing that the protein interaction between OSCP and CypD was significantly reduced by 45% in the phosphoresistant S191A group, averaging 0.65 ± 0.04 vs. 1.00 ± 0.07 in WT group (Fig. 2b), whereas the ...
3a-c). Wound healing and transwell assays showed that the migration ability of gastric cancer cells significantly decreased upon CLDN6 knockdown (Fig. 3d-g). Together, these results suggest that silencing CLDN6 expression inhibited the proliferation and invasion abilities of GC in vitro. Fig. 3:...
Cells were detached from culture plates with non-enzymatic cell dissociation solution (Sigma-Aldrich) and used for functional assays. Ex vivo whole lung imaging and confocal microscopy analysis Whole lung imaging was performed as previously described.19 Briefly, PC3 and MDA-MB-231 (5 × 105) ...
blood mononuclear cells (PBMC)Achieving a humanization ratio of over 40% within 3 weeks.Predominantly T cells, accounting for over 90%.Tumor immunology research; anti-GvHD drug research; infectious disease research; gene therapy; drug target research without cross-reactivity; immunogenicity assays. ...