readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Both experts and non-experts in the scientific community who wish to study cell adhesion molecules and diagnostics will find Integrin and Cell Adhesion Molecules: Methods and Protocols authoritative, easily accessible,...
Chemoresistance is a major hurdle in anti-cancer therapy. Growing evidence indicates that integrin-mediated cell adhesion to extracellular matrix plays a major role in chemoresistance. However, the underlying mechanisms are not fully understood. We have
EpCAM (epithelial cell adhesion molecule; CD326 (cluster of differentiation 326)) was originally identified as a novel tumour-specific cell surface antigen after immunisation of mice with cancer cells in 1970s, and was later defined as a cell–cell adhesion molecule6,7. EpCAM is a type I tr...
day 6 half of the supernatant is removed, centrifuged at 200gfor 5min, resuspended in fresh RPMI-1640 complete medium supplemented with 20ng/mL GM-CSF and 50nM β-mercaptoethanol and added back to the original culture (seeNotes 13 and 14). Cells can be harvested on day 7 (seeNote 15)...
(Fig.1e and f). Glomerular cell types showed regulations of extracellular matrix molecules, cell adhesion, cell proliferation, etc., and cellular responses to mechanic stress, cytokines, and blood pressure. PT cell types showed multiple metabolic processes such as biological oxidations, and responses...
Technical Notes and Product HighlightsPage Contents Cell Enumeration and Cell Proliferation Assays for Animal Cells Detection and Enumeration Assays for Microorganisms and Viruses Countess Automated Cell Counter Premo FUCCI Cell Cycle Sen...
S8C, D). By comparing myeloma cells in pre-treated samples with post-treated samples both in responders and non-responders, we found genes and pathways involving TGFβ signaling and cell adhesion were downregulated after treatment, while stress-associated genes and pathways (e.g., apoptosis, ...
The non-cellular components of TME include the extracellular matrix (ECM) and the signaling molecules produced by the cancer cells and stromal cells. Pancreatic ductal adenocarcinoma (PDAC), which accounts for > 90% of all pancreatic cancer cases, is one of the solid tumor types known to ...
BMC Cancer (2024) Cell adhesion molecules’ altered profile in benign and malignant salivary gland tumors. The paradigm of beta4-integrin, desmoglein-2, ICAM-1 and CD44s Dimitrios Andreadis Athanasios Poulopoulos Dimitrios Antoniades Journal of Biological Research-Thessaloniki (2020)Download...
1b). We speculated that the inefficient fluorescence labeling of GRAPHIC in cancer cells was due to the unstable cell–cell adhesion of cancer cells. To achieve efficient optical labeling of cell–cell interactions involving cancer cells, sGRAPHIC was conceived using a combination of GPI-anchored N...