Assure ability to implement triage activities based on public health guidance including at the facility and using remote (i.e., phone, internet-based) methods where appropriate to minimize demand on the health care system. Ensure that negative-pressure airborne infection isolation rooms are available ...
Assure ability to implement triage activities based on public health guidance including at the facility and using remote (i.e., phone, internet-based) methods where appropriate to minimize demand on the health care system. Ensure that negative-pres...
Both femora and tibia of mice were isolated and the BM was harvested by either flush or spin isolation. At least one tenth of the total cells were transferred to a new tube, centrifuged for 5 min at 300 xg and resuspended in a 1:1 dilution of CATCH buffer (25 mM HEPES, 3mM EDTA, ...
Assureabilityto implement triage activities based on public health guidance including at the facility and using remote (i.e., phone, internet-based) methods where appropriate to minimize demand on the health care system. Ensure that negative-pressure airborne infection isolation rooms are available and...
Ensure that negative-pressure airborne infection isolation rooms are available and functioning correctly and are appropriately monitored for airflow and exhaust handling. Assess availability of personal protective equipment (PPE) and other infection prevention and control supplies (e.g., hand hygiene suppli...
Ensure that negative-pressure airborne infection isolation rooms are available and functioning correctly and are appropriately monitored for airflow and exhaust handling. Assess availability of personal protective equipment (PPE) and other infection prevention and control supplies (e.g., hand hygiene suppli...
For analysis of CD8+ T cell priming in tdLN, OT-I CD8+ T cells were isolated from spleens of OT-I x CD45.1 donor mice using the naive CD8+ T cell isolation kit (Miltenyi Biotec). 1x103 cells were adoptively transferred intravenously (i.v.) in sterile PBS into recipient mice 6h be...
Put on a clean isolation gown upon entry into the patient room or area. Change the gown if it becomes soiled. Remove and discard the gown in a dedicated container for waste or linen before leaving the patient room or...
RNA isolation and PCR Total RNA was extracted using TRI reagent (Sigma) as described earlier.49 cDNA was prepared using the cDNA synthesis kit from Applied Biosystems (Waltham, MA, USA) following the manufacturer's protocol. For each reaction 100 μg of total RNA was used. The cDNA ...
Put on a clean isolation gown upon entry into the patient room or area. Change the gown if it becomes soiled. Remove and discard the gown in a dedicated container for waste or linen before leaving the patient room or care area. Disposable gowns should be discarded after use. Cloth gowns ...