Download all Video S1. In vitro proplatelet formation of WT BM-derived MKs using time-lapse microscopy, related to Figure 3 Video S2. In vitro proplatelet formation of RhoA BM-derived MKs using time-lapse microscopy, related to Figure 3 Video S3. In vitro proplatelet formation of Cdc42 ...
For analysis of CD8+ T cell priming in tdLN, OT-I CD8+ T cells were isolated from spleens of OT-I x CD45.1 donor mice using the naive CD8+ T cell isolation kit (Miltenyi Biotec). 1x103 cells were adoptively transferred intravenously (i.v.) in sterile PBS into recipient mice 6h be...
In vitro BM MK isolation and differentiation BM cells were obtained from femur and tibia by flushing, and lineage depletion (Lin−) was performed using CD16/CD32+, Gr1+, B220+ and CD11b+ antibodies (Biolegend). Lin− cells were cultured in 2.6% nutrient-supplemented StemPro medium with...
Afterasking retired medical professionals to return to work, the government has alsoput out a call for 250,000 volunteers to support the NHS(NationalHealth System). You can apply to be a community response volunteer, collecting anddelivering ...
A5E36770603-AB 3.3.3 Mounting and connecting the HMI device 3.3 Connecting the device Connecting the power supply NOTICE Safe electrical isolation For the 24 V DC supply, use only power supply units with safe electrical isolation in accordance with IEC 60364-4-41 or HD 384.04.41 (VDE 0100,...
Predicting perceived isolation among midlife and older LGBT adults: The role of welcoming aging service providers Gerontologist, 58 (5) (2018), pp. 904-912, 10.1093/geront/gnx092 Google Scholar Cited by (9) Special Report from the CDC: Suicide rates, sodium nitrite-related suicides, and onlin...
The structure of the P7 peptide in isolation was determined by NMR. The peptide was fully assigned using standard homonuclear experiments, and the NOESY was used to generate 440 unique distance restraints. The RMSD of the final water-refined structures is 0.37 Å over all backbone atoms, and...