CD74基因调控CXCR4抑制肾癌786-O细胞的侵袭作用
【摘要】目的 探讨抑制人CD74基因后影响肾癌786-O细胞侵袭能力的作用机制.方法 通过慢病毒lentivirus-CD74转染肾癌786-O细胞后,MTT法观察细胞活力变化,Transwell实验观察细胞侵袭能力的变化,应用Westernblot技术检测CD74、CXCR4蛋白的变化情况.结果 抑制CD74蛋白表达水平后肾癌786O细胞活力降低,侵袭能力降低,同时伴有CXCR...
CXCR4侵袭目的探讨抑制人CD74基因后影响肾癌786-O细胞侵袭能力的作用机制.方法通过慢病毒lentivirus-CD74转染肾癌786-O细胞后,MTT法观察细胞活力变化,Transwell实验观察细胞侵袭能力的变化,应用Westernblot技术检测CD74,CXCR4蛋白的变化情况.结果抑制CD74蛋白表达水平后肾癌786-O细胞活力降低,侵袭能力降低,同时伴有CXCR4...
Background Although macrophage migration inhibitory factor ( MIF ) has been demonstrated to mediate cardioprotection in ischemia/reperfusion injury and antagonize fibrotic effects through its receptor, CD 74, the function of the soluble CD 74 receptor ectodomain ( sCD 74) and its interaction with circ...
HIV gp120; CC chemokine receptor 5 (CCR5; CD195); CXC chemokine receptor 4 (CXCR4; NPY3R) In vitro and cell assay studies identified a dual CD4-heparin sulfate peptide (mCD4-P3YSO3) that could help treat HIV. Further details on the research, next steps and licensing status are ...
MIF promotes B cell chemotaxis through the receptors CXCR4 and CD74 and ZAP-70 signaling. J Immunol. 2014;192:5273-84. (Block)Klasen C, Ohl K, Sternkopf M, Shachar I, Schmitz C, Heussen N, et al. MIF promotes B cell chemotaxis through the receptors CXCR4...
Activation of the JNK signalling pathway by macrophage migration inhibitory factor (MIF) and dependence on CXCR4 and CD74 . Cell Signal 2011; 23 :135–144.Lue, H., Dewor, M., Leng, L., Bucala, R., and Bernhagen, J. (2011). Activation of the JNK signalling pathway by macrophage ...