CD74占据MHCII的结合槽，防止内质网中内源性肽分子与MHCII结合。CD74的形成与跨膜区域和三聚体化结构域（C端细胞外区域中的氨基酸残基134-208）有关。CD74还参与MHCI呈递抗原，激活CD8+T细胞，引发MHCI介导的细胞毒性T细胞反应。此外，CD74还参与MIF信号传导，与CXCR2、CXCR4或CXCR7形成复合物，激活多条信号通路...
CD74基因调控CXCR4抑制肾癌786-O细胞的侵袭作用
提示CD74在肺腺癌的发生发展中起重要作用。细胞外MIF是一种炎性细胞因子,与CD74、CXC趋化因子受体(CXCR)2、CXCR4和CXCR7相互作用,在许多肿瘤模型中诱导血管生成、增殖和间质-上皮转化。在肺Ⅱ型上皮细胞中,MIF与CD74结合,与细胞增殖...
MTT法观察细胞活力变化,Transwell实验观察细胞侵袭能力的变化,应用Westernblot技术检测CD74、CXCR4蛋白的变化情况.结果 抑制CD74蛋白表达水平后肾癌786O细胞活力降低,侵袭能力降低,同时伴有CXCR4蛋白的表达降低.结论 通过慢病毒转染抑制CD74的表达可以抑制肾癌细胞的侵袭能力,CD74基因可能是通过调控CXCR4蛋白的变化影响细胞...
MIF receptor profiling of resting primary human CD4 + T cells via flow cytometry revealed high surface expression of CXCR4, while CD74, CXCR2 and ACKR3/CXCR7 were not measurably expressed. However, CD4 + T cells constitutively expressed CD74 intracellularly, which upon T-cell activation was ...
Recent studies have identified CXCR2 and CXCR4 as co-receptors for CD74 where MIF binding to CD74 complexes contributes to MIF-mediated monocyte chemotaxis and the induction of Akt signaling, respectively (7,8). Increased CD74 surface expression has been re...
[11]. Antibody blockade experiments revealed that atherogenicleukocyte recruitmentby MIF is dependent on CD74. An overlap between the MIF/CD74 and MIF/CXCR2 or MIF/CXCR4 signaling pathways has been suggested[11]. InT cells, both CD74 andCXCR4were required for MIF-stimulated AKT signaling, ...
Gene expression analyses using microarray and RT - qPCR technology revealed a 4-fold upregulation of several interferon-induced genes upon co-treatment of myofibroblasts with sCD 74 and MIF (Ifi44: P=0.011; Irg1: P=0.022; Clec4e: P=0.011). Furthermore, Western blot analysis confirmed the ...
Fig. 4. CD74 as a receptor for the cytokines macrophage migration inhibitory factor (MIF) and MIF-2. MIF and MIF-2 bind to the extracellular domain of CD74. In order to induce signalling, CD74 requires co-receptors like CD44 or CXCR4. Activation of several downstream signalling pathways ...
CD74 interaction with CD44 and binding to the proinflammatory cytokine macrophage migration-inhibitory factor (MIF). CD74 also plays a role in the maturation of follicular B-cells and accumulation of marginal zone B-cells. CD74 has also been demonstrated to interact with CXCR2 and CXCR4. ...