肾脏中,单核细胞/巨噬细胞群定义为 CD11b+和Ly6Chigh、Ly6Cintermediate(int)或Ly6Clow。所有三个群体都以低水平存在于正常肾脏中。在炎症模型中,会随着炎症程度的改变,逐渐改变。 10. 小鼠睾丸组织 11. 脂肪组织 小鼠脂肪组织巨噬...
Further studies using Fc receptor blockers disclosed that this population was a renal macrophage subset which was different from other macrophages by its intermediate expression of CD45 and CD11b. These CD45intCD11bint macrophages were further characterized as F4/80+MHCII+Ly6C cells comprising 40%...
Purity of isolated neutrophils was verified by flow cytometry using CD11b, Ly6C, and Ly6G markers. For measurement of ROS (superoxide) production by luminol-based assay (49, 50), bone marrow cells were plated in a black 96-well plate (SPL Life Sciences) at 106 cells per well in IMDM ...
(R17217, eBioscience), CD34 (RAM34, eBioscience), CD24 (M1/69, BioLegend), CD43 (1B11, BioLegend), VCAM-1 (429, BioLegend), CD90 (HIS51, eBioscience), Ly6C (HK1.4, eBioscience), CD127 (A7R34, eBioscience), Ly6D (49-H4, BioLegend), CD19 (ID3, eBioscience), CD144 (...
Inflammation switches the differentiation program of Ly6Chi monocytes from antiin- flammatory macrophages to inflammatory dendritic cells in the colon. J. Exp. Med. 209, 139–155 (2012). 15. Zigmond, E. et al. Macrophage-restricted interleukin-10 receptor defi- ciency, but not IL-10 ...
inflammationPLoS biologyDecember 1, 2023Yan T, Wang R, Yao J, Luo M.fromBioLegendand the dilutions were1:200: anti-F4/80-PE (Cat:#123109), anti-CD45-APC/Cyanine7 (Cat:#103115), anti-CD11b-FITC (Cat:#101206), anti-Ly6C-BrilliantViolet605 (Cat:#128035),anti-CD206 (MMR)-PE/...
We also identified the CD45intCD11bint MPC subtype in human kidney. We conclude that CD45intCD11bint F4/80+MHCII+CX3CR1+Ly6C-population represent a unique subset of MPCs found in both mouse and human kidneys. Future studies will further characterize their role in kidney health and disease...