Recent thymic emigrants (RTE) and stem cell-like memory T cells (TSCM) can also replenish a T cell pool. In this study we formally test whether CD44v.low cells are the same population as RTE and TSCM. Our data show that, in contrast to RTE, CD44v.low cells express high levels of ...
Easily and efficiently isolate highly purified mouse naïve CD4+ T cells (CD4+CD44lowCD62Lhigh) from mouse splenocytes by immunomagnetic negative selection with the EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit. When using single-cell suspensions from other tissue types, this kit may ...
在C57BL/6小鼠中,记忆T细胞高表达 CD44,这是一种与透明质酸结合的粘附分子,因此可以与具有中等/弱CD44表达的初始T细胞区分开来。 小鼠CD4 + 和 CD8 + T 细胞中可分出以下四个初始/记忆 T 细胞亚群:CD44 int/low CD62L + naïve...
结果发现在pLN和SLO,InsC-ChgA和InsB特异性CD4+T细胞的比例均显著高于InsC-IAPP CD4+T细胞。然而,仅在第二周,就有80%和60%的InsC-ChgA特异性CD4+T细胞在pLN和SLO处于CD44+活化状态。相反,大部分InsC-IAPP和InsB特异性CD4+T细胞则...
结果:小鼠脾脏淋巴细胞分离后直接做流式检测,CD3+CD4+ 细胞占 19畅09%,其中 98畅61%的细胞为 CD44+,68畅71%为CD62L+。 体外培养后 CD3+CD4+ 细胞占8畅96%,其中71畅82%为CD44+,11畅27%为CD62L+。 哮喘小鼠脾脏淋巴细胞分离后直接做流式检测,CD3+CD4+ 细胞占 20畅33%,其中 97畅72%的细胞为 CD44...
Specialized subpopulations of CD4+ T cells survey major histocompatibility complex class II–peptide complexes to control phagosomal infections, help B cells, regulate tissue homeostasis and repair or perform immune regulation. Memory CD4+ T cells are positioned throughout the body and not only protect...
活化标志物CD69、CD25和CD44在hCD4+T细胞中的表达增加,证实了与对照组相比,这些细胞在用WT铜绿假单胞菌PA14预消化的DAPT-麦胶蛋白刺激的共培养中具有更强的活化表型。这与一系列细胞因子和趋化因子的水平升高有关,包括当类器官单层分别用lasB△/△、DAPT-麦胶蛋白或WT铜绿假单胞菌PA14上清酶预消化的DAPT-麦胶...
活化标志物CD69、CD25和CD44在hCD4+T细胞中的表达增加,证实了与对照组相比,这些细胞在用WT铜绿假单胞菌PA14预消化的DAPT-麦胶蛋白刺激的共培养中具有更强的活化表型。这与一系列细胞因子和趋化因子的水平升高有关,包括当类器官单层分别用lasB△/△、DAPT-麦胶蛋白或WT铜绿假单胞菌PA14上清酶预消化的DAPT-麦胶...
The initial thymocyte population displays c-Kit, the receptor for stem cell growth factor, and subsequently expresses CD44, an adhesion molecule, and then CD25, the Alpha-Chain of the IL-2 Receptor. During this period, the cells proliferate b...
Why Isolate CD4+/CD62L+/CD44lowNaïve T cells? Mouse CD4+T cells develop in the thymus and are released as naïve T cells that also express CD62L and low levels of CD44. These cells differentiate into subsets of specialized effector T lymphocytes in response to distinct environmental ...