因此,研究人员试图提高工程细胞的功能和存活率。通过添加细胞因子发现,与IL-2相比,在IL-7/IL-15中培养的CD4+T细胞显示TSCM样细胞(CD44−CD62+Sca-1hi)的频率显著增加(图4a)。 在动物实验中,将两种细胞因子培养条件下(IL-7/IL-15和IL-2)的TCR工程细胞...
Wen. 2008. T cell receptor-mediated activation of CD4+CD44hi T cells bypasses Bcl10: an implication of differential NF-kappaB dependence of naive and memory T cells during T cell receptor-mediated responses. J. Biol. Chem. 283:24392-24399. doi:10.1074/jbc.M802344200...
在C57BL/6小鼠中,记忆T细胞高表达 CD44,这是一种与透明质酸结合的粘附分子,因此可以与具有中等/弱CD44表达的初始T细胞区分开来。 小鼠CD4 + 和 CD8 + T 细胞中可分出以下四个初始/记忆 T 细胞亚群:CD44 int/low CD62L + naïve...
Easily and efficiently isolate highly purified mouse naïve CD4+ T cells (CD4+CD44lowCD62Lhigh) from mouse splenocytes by immunomagnetic negative selection with the EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit. When using single-cell suspensions from other tissue types, this kit may ...
500 μL (CD4+ T 细胞) 染色buffer重悬(CD4-PE, CD44- PE/Cyanine7, CD62L -APC)。 混匀,冰箱中孵育30 min (2℃-8℃)。 准备单阳管,调补偿。 4℃,500 g/10 min离心。弃上清。 1 mL FACS buffer洗。4℃,500 g/10 min离心。弃上清。
结果:小鼠脾脏淋巴细胞分离后直接做流式检测,CD3+CD4+ 细胞占 19畅09%,其中 98畅61%的细胞为 CD44+,68畅71%为CD62L+。 体外培养后 CD3+CD4+ 细胞占8畅96%,其中71畅82%为CD44+,11畅27%为CD62L+。 哮喘小鼠脾脏淋巴细胞分离后直接做流式检测,CD3+CD4+ 细胞占 20畅33%,其中 97畅72%的细胞为 CD44...
Specialized subpopulations of CD4+ T cells survey major histocompatibility complex class II–peptide complexes to control phagosomal infections, help B cells, regulate tissue homeostasis and repair or perform immune regulation. Memory CD4+ T cells are positioned throughout the body and not only protect...
500 μL (CD4+ T 细胞) 染色buffer重悬(CD4-PE, CD44- PE/Cyanine7, CD62L -APC)。 混匀,冰箱中孵育30 min (2℃-8℃)。 准备单阳管,调补偿。 4℃,500 g/10 min离心。弃上清。 1 mL FACS buffer洗。4℃,500 g/10 min离心。弃上清。
Once the cells stop expressing c-Kit and markedly reduce CD44 expression, they begin to rearrange their TCR genes. The earliest stages of T-cell lineage commitment also witness expression of two transcription factors, Ik1 and GATA3 (GATA Bindi...
The MojoSort™ Mouse CD4 Naïve T cell Isolation Kit was used to isolate cells from C57BL/6 mouse spleen. Top: CD4+T cells after isolation. Middle: CD4+ Naïve T cells after isolation, defined by the CD62L (clone: MEL-14) PE and CD44 (clone: IM7) FITC profile. Bottom: cells...