McElwee KJ, Freyschmidt-Paul P, Hoffmann R, Kissling S, Hummel S, Vitacolonna M, Zoller M (2005) Transfer of CD8(+) cells induces localized hair loss whereas CD4(+)/CD25(-) cells promote systemic alopecia areata
Naturally arising CD25+ CD4+ regulatory T cells (TR) play an important role in the prevention of autoimmunity. TCR specificity is thought to play a critical role in TR development and function, but the repertoire and specificity of TR TCRs remain largely unknown. We find by sequencing of TRAV...
Nonactivated (resting) CFSE-labeled cells did not divide and typically contained a small percentage of cells expressing CD25 and CD69 (Figure 5A). Either in the presence or absence of AZT, killing of resting CFSE-labeled CD4 T cells was robust (Figure 5B, columns 4 and 5, and 16 and ...
目的:观察肝癌患者手术治疗前后CD4+CD25+Tregs的变化。探讨 手术治疗与患者免疫之间的关联性及意义。 方法:收集2010年12月.2011年12月在衡水市哈励逊医院住院的肝 细胞肝癌患者,男15例,女5例;正常对照组20例,来自衡水市哈励逊 健康体检者。 入组标准:l、肿瘤单发<5cm,或病灶小于3个,每个病灶均<3cm。
Cell suspensions were centrifuged and stained with the following with antibodies at 0.5 µg ml−1 per antibody: APC anti-mouse CD3, Pacific Blue anti-mouse CD4, BV785 anti-mouse CD8, PerCP anti-mouse CD25, FITC anti-mouse F4/80, Pe-Cy7 anti-mouse CD11c, PE anti-mouse NK1.1...
A minus sign preceding correlation coefficient indicates negative correlation. d day, DC dendritic cell, iTreg induced Treg, LPS lipopolysaccharide, MSC mesenchymal stem cell, MSC-DC MSC-treated DC, MLR mixed lymphocyte reaction, ns not significant, TC allogeneic CD4+CD25− T cells Full size ...
Gating strategies excluded dead cells, clumps, and debris and were set based on fluorescence minus one (FMO) and unstained samples for splenocytes and unstained samples for SC cells. All CD4 T-cell populations were gated from lymphocytes (SSC-A vs FSC-A), live cells (Fixable Viability Dye...
www.nature.com/scientificreports OPEN Multiple sclerosis patients have reduced resting and increased activated CD4+CD25+FOXP3+T regulatory cells Nirupama D. Verma1,2*, Andrew D. Lam1,2, Christopher Chiu1,2, Giang T. Tran1,2, Bruce M. Hall1,2 & Suzanne J....
When autologous CD25− PBMC fractions were incubated with tumor cells from patients (n = 6) in vitro, there was consistent strong induction and then expansion of cells with the CD4+CD25+FoxP3+ phenotype of classic “natural” Treg cells. This population was confirmed to be suppressive in ...
Neuropsychiatric Disease and Treatment Dovepress open access to scientific and medical research Open Access Full Text Article Original Research Shen-Qi-Jie-Yu-Fang exerts effects on a rat model of postpartum depression by regulating inflammatory cytokines and CD4+CD25+ regulatory T cells Jingya Li1,...