4b–d). This protection was antigen-specific, as mice receiving 3 × 106 activated polyclonal CD4+ T cells not expressing the CLTCH129>Q-specific TCR had tumor growth comparable to untreated mice. None of the five protected mice receiving a subsequent tumor challenge 30 d after initial...
Discussion Activated but non-proliferative T cells contribute to distinct CD4+ T cell signature associated with BTB infection. Despite having the same overall numbers of lymphocytes, CD4+ T cells from TB infected cattle exhibit distinct transcriptomic expression profiles, as evident by the clear ...
Consistently, the intense bands of phospho-PKA substrates indicated that the PKA/cAMP pathway was clearly activated in oligodendrocytes of Miga2−/− mice and was downregulated by depleting CD4+ T cells (Figure S5K). As the only detectable adenosine receptor, A1 in oligodendrocytes was further...
To determine whether Treg cells also suppress acquisition of cytotoxic potential by CD4+ T cells, we activated purified human naive CD4+ T cells and co-cultured them with different ratios of autologous Treg cells. Low numbers of Treg cells (1:10 Treg/Teff cells) significantly suppressed GzmB ...
Tumor or healthy lung infiltrating T cells were activated with 2 μg/ml soluble anti-human CD3 (OKT3; Biolegend) and 2 μg/ml anti-human CD28 (CD28.2; Biolegend) in the presence of r-activin-A (50 ng/ml) or PBS for 3–5 days. Activin-A or PBS-treated CD4+ T cells ...
Impaired induction of c-fos/c-jun genes and of transcriptional regulatory proteins binding distinct c-fos/c-jun promoter elements in activated human T cells during aging. Cell Immunol. 1997; 175:41–50. [PubMed: 9015187] 53. Kurtulus S, Tripathi P, Opferman JT, Hildeman DA. Contracting ...
6a). Coherent activation or repression activity by either JunB-BATF or JunB-Fosl2 collaborations are well correlated with actual changes in gene expression in Th17 relative to Th0 cells (i.e., genes activated by both TFs are upregulated in Th17 cells) supporting dominant regulatory action by ...
Indeed, when activated with aCD3/aCD28, CD4 þ CD103 þ TRM demonstrated accelerated kinetics for IFNg production when compared with blood-derived CD45RA À memory T-cells (TM) (Figure 6b). Although after only 2 h of stimula- tion, on average 17% of CD4 þ CD103 þ TRM ...
International Journal of Molecular Sciences Article Induction of a Regulatory Phenotype in CD3+ CD4+ HLA-DR+ T Cells after Allogeneic Mixed Lymphocyte Culture; Indications of Both Contact-Dependent and -Independent Activation Anne Louise Schacht Revenfeld 1,*, Rikke Bæk 1, Malene Møller ...
First, we examined the effect of DPV576 on expression of TRPV1 on non-activated CD4+ T cells. As shown in Figure 1A–E, exposure of CD4+ T cells to DPV576 in the absence of T cell activation did not have a significant effect on the expression of TRPV1 on CD4+ T cells. Second,...