结果发现在pLN和SLO,InsC-ChgA和InsB特异性CD4+T细胞的比例均显著高于InsC-IAPP CD4+T细胞。然而,仅在第二周,就有80%和60%的InsC-ChgA特异性CD4+T细胞在pLN和SLO处于CD44+活化状态。相反,大部分InsC-IAPP和InsB特异性CD4+T细胞则...
结果:小鼠脾脏淋巴细胞分离后直接做流式检测,CD3+CD4+ 细胞占 19畅09%,其中 98畅61%的细胞为 CD44+,68畅71%为CD62L+。 体外培养后 CD3+CD4+ 细胞占8畅96%,其中71畅82%为CD44+,11畅27%为CD62L+。 哮喘小鼠脾脏淋巴细胞分离后直接做流式检测,CD3+CD4+ 细胞占 20畅33%,其中 97畅72%的细胞为 CD44...
活化标志物CD69、CD25和CD44在hCD4+T细胞中的表达增加,证实了与对照组相比,这些细胞在用WT铜绿假单胞菌PA14预消化的DAPT-麦胶蛋白刺激的共培养中具有更强的活化表型。这与一系列细胞因子和趋化因子的水平升高有关,包括当类器官单层分别用lasB△/△、DAPT-麦胶蛋白或WT铜绿假单胞菌PA14上清酶预消化的DAPT-麦胶蛋...
Easily and efficiently isolate highly purified mouse naïve CD4+ T cells (CD4+CD44lowCD62Lhigh) from mouse splenocytes by immunomagnetic negative selection with the EasySep™ Mouse Naïve CD4+ T Cell Isolation Kit. When using single-cell suspensions from other tissue types, this kit may ...
T cell receptor-mediated activation of CD4+CD44hi T cells bypasses Bcl10: an implication of differential NF-kappaB dependence of na?ve and memory T cells during T cell receptor-mediated responses. Zeng Hu,Chen Yuhong,Yu Mei,Xue Liquan,Gao Xiang,Morris Stephan W,Wang Demin,Wen Renren. ...
小鼠CD4 + 和 CD8 + T 细胞中可分出以下四个初始/记忆 T 细胞亚群:CD44 int/low CD62L + naïve、CD44 high CD62L + CM、CD44 high CD62L - EM 和 CD44 int/low CD62L- EMRA。 准确检测体内增殖情况对于跟踪T细胞反应的...
观察到包括CD28、CD69、CD44和ICOS在内的T细胞活化相关标志物在PD-1 CD45RO DPT细胞中上调，表明PD-1 CD45RO DPT细胞可能表现出活化表型。这种在 SPT 细胞中表达和激活的 PD-1 表型已在肺癌中报道。 然后，通过比较 SPT、DPT 和 DNT 细胞之间免疫耗竭因子的表达水平。几乎所有免疫衰竭标志物在 PD-1CD45...
Specialized subpopulations of CD4+ T cells survey major histocompatibility complex class II–peptide complexes to control phagosomal infections, help B cells, regulate tissue homeostasis and repair or perform immune regulation. Memory CD4+ T cells are positioned throughout the body and not only protect...
活化标志物CD69、CD25和CD44在hCD4+T细胞中的表达增加,证实了与对照组相比,这些细胞在用WT铜绿假单胞菌PA14预消化的DAPT-麦胶蛋白刺激的共培养中具有更强的活化表型。这与一系列细胞因子和趋化因子的水平升高有关,包括当类器官单层分别用lasB△/△、DAPT-麦胶蛋白或WT铜绿假单胞菌PA14上清酶预消化的DAPT-麦胶...
The initial thymocyte population displays c-Kit, the receptor for stem cell growth factor, and subsequently expresses CD44, an adhesion molecule, and then CD25, the Alpha-Chain of the IL-2 Receptor. During this period, the cells proliferate b...