Objective: To investigate the changes of gene expression in CD34 hematopoietic stem and progenitor cells (HSPCs) under different growth environments. Methods: Umbilical cord blood mononuclear cells (UCB MNCs) were cultured in static and stirred systems. After 7 days of culture, CD34 cells were ...
The hematopoietic and non-hematopoietic stem/progenitor cells harvested directly from the bone marrow (BM) or G-CSF mobilized peripheral blood were demonstrated to play an important role in regeneration of damaged organs ( 1, 2 ). Here, we asked if the stroke-...
Hematopoietic Stem and Progenitor Cells Species Human Cell and Tissue Source Cord Blood Donor Status Normal Purity The purity of CD34+cells is ≥ 90% by flow cytometry. References: 1. "Human PubMed Reference:". https://www.ncbi.nlm.nih.gov/pubmed?linkname=gene_pubmed&from_uid=947 ...
We found 33 miRNAs expressed in CD34+ hematopoietic stem-progenitor cells (HSPCs) from normal human bone marrow and mobilized human peripheral blood stem cell harvests. We then combined these data with human HSPC mRNA expression data and with miRNA-mRNA target predictions, into a previously ...
using uniform, superparamagnetic polymer beads coated with a primary monoclonal antibody specific for the CD34 membrane antigen predominantly expressed on human hematopoietic progenitor cells and endothelial progenitor cells. The isolated cells are poured off into a new tube, and are cryogenically preserved...
Stem cellsProgenitor cellsTransfection of Jurkat and 293T cells does not show significant impacts on susceptibility to viral transduction. A representative flow cytometry plot of Jurkat cells transfected with pmCherry-C1 plasmid DNA and subsequently virally transduced with ZsGreen pnl4.3 HIV-1-based ...
CD34 is routinely used to identify and isolate human hematopoietic stem/progenitor cells (HSPCs) for use clinically in bone marrow transplantation, but its function on these cells remains elusive. Glycoprotein ligands on HSPCs help guide their migration to specialized microvascular beds in the bone mar...
Hematopoietic Stem and Progenitor Cells Species Human Application Cell Culture, Expansion Brand StemSpan Area of Interest Stem Cell Biology, Transplantation Research Formulation Category Serum-Free View More Data Figures Table 1.HSC Expansion Culture with CD34+Human Cord Blood Cells Cultured in StemSpan™...
CD34 is highly expressed on hematopoietic progenitors, as well as on endothelial cells, brain, and testis. Staining for CD34 has been used to measure angiogenesis, which reportedly predicts tumor recurrence.Hematopoietic Stem Cell MarkerEndothelial MarkersTumor biomarkers Size:50ul Concentration:1mg/ml ...