ISCT标准:CD73+/CD90+/CD105+>95%,CD34-/CD45-/HLA-DR-<2%FDA要求:细胞活性>80%,传代次数≤5代中国药典:每批次细胞需进行成骨/成脂分化验证2. 关键质控节点① 细胞溯源:需提供STR基因分型报告② 微生物检测:涵盖细菌、真菌、支原体、内毒素③ 功能验证:迁移实验(Transwell小室迁移数>200/视野)免疫抑制率...
Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. Properties Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the ...
Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. The calculated molecular mass of human CD34 is 41 kDa, various forms with different molecular weights may be ...
Gene expression of CD34, CD45, CD73 and CD105 in rat MSCs, rat bone marrow, mouse MSCs and mouse bone marrow.Goichi YanaiTakashi HayashiQi ZhiKaiChiang YangYasumasa ShirouzuTakashi ShimabukuroAkihito HiuraKazutomo InoueShoichiro Sumi
Gp105-120, My10, Mucosialin Isotype Mouse IgG2a, κ Ave. Rating Submit a Review Product Citations 33 publications Human peripheral blood leukocytes stained with CD45 (HI30) FITC and 561 APC (top) or mIgG1, κ APC isotype control (bottom). Data was generated by gating on liv...
ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29,CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) ...
关键词:急性白血病;CDl33;CD34:流式细胞术;预后 英文摘要 Leukemiaand 133andCD34inAcute The ofCD Expression itsClinical Significance ABSTRACT in evaluatethe of CD34 CDl33(ACl33)and objeetive:To expression to thecorrelationofthe acute explore expression leukelIlia(AL)patients,and andALFAB of tofurth...
CD surface markers have been used to isolate different populations of mBMSCs with differences in their functions. For example: PDGFRα+ Sca-1+ CD45− TER119− (Morikawa et al., 2009), CD34+/CD73+ (Akiyama et al., 2012), CD105+ (Goussetis et al., 2005), CD271+/CD90+/CD106...
结果: 1.人骨髓MSC贴壁层的建立及鉴定体外培养12d左右,MSC融合达70%～80%,呈漩涡状生长.细胞传至第三代,其表面表达CD90,CD105,CD166,CD29,CD44,CD13和CD49e,不表达CD14,CD34,CD31,CD45和HLA-DR. 2.体外共培养后各份CD34+细胞的归巢能力变化脐血CD34+细胞分选富集的纯度为(98.25±0.93)%,实验组在共...
表达CD29,CD44,CD73,CD90,CD105,不表达 CD34,CD45 以及 CD11b,CD14,CD19,CD79a,CD117和HLA-DR分子,符合国际细胞治疗学会制定的MSCs标准,为MSCs中的一员... 项炳羽 - 《浙江大学》 被引量: 0发表: 2017年 汉黄芩素上调小鼠骨髓Lin-细胞Pecam1基因表达 [目的]探讨汉黄芩素对小鼠骨髓Lin-细胞增殖、分化...