It was demonstrated that CD34 + CD38 - subpopulation without CD123 and CD44 might be held responsible for LSC and correlated with an imbalance of immune cell subsets in AML.QIANSHAN TAOQING ZHANGHUIPING WANGHAO XIAOMEI ZHOULINLIN LIUHUI QIN...
目的探讨急性髓系白血病(AML)患者CD34、CD123、CD38的表达及其临床意义。 方法收集2014年2月至2015年7月中南大学湘雅医院164例AML患者,用流式细胞术检测患者细胞免疫分型。根据CD34、CD38、CD123的表达情况,将患者分成阳性组与阴性组,对两组的临床资料及骨髓象进行比较分析。 结果164例AML患者中,CD34阳性102例,阳性...
We introduce and validate a post-treatment approach of leukemic stem cell (LSC) assessment for prediction of outcome. LSC containing CD34+CD38− fractions were measured using flow cytometry in an add-on study of the HOVON102/SAKK trial. Predefined cut-off levels were prospectively evaluated to ...
CD33 expression is usually lower in immature leukemic cells (CD34+/CD38− or CD34+/CD38−/CD123+): particularly, it was recently shown that leukemic CD34+/CD38− cells display higher CD33 levels than the normal CD34+/CD38− counterpart [95]. In line with this observation, Taussig and...
【方法】1.以CD34+CD38’CDl23+作为识别白血病干细胞(1eukemiastemcells,LSC)的免疫表型,利用流式细胞术检测白血病患者骨髓中是否存在免疫表型为CD34+CD38一CDl23+的LSC;2.根据WHO分型标准将白血病患者分为急性髓细胞白血病(acutemyeloidleukemia,AML)组、急性淋巴细胞白血病(acutelymphocyticleukemia,ALL)组、急性双...
目的 从白血病KGla细胞中分选CD34+ CD38-干细胞并研究其生物学特性.方法 免疫磁珠法分选CD34+ CD38-细胞,流式细胞术分析细胞表面膜抗原,细胞周期,甲基纤维素培养体系观察其克隆性;以HL60,K562,CD34+ CD38+细胞为对照,甲基偶氮唑蓝法检测柔红霉...
In contrast, IL-3Rα expression was very low or undetectable in the total CD34+ population and in the more primitive CD34+CD38− cells of normal bone marrow (Figure 1B; Table 1). Moreover, IL-3Rα staining on CD34+CD38− cells of 4 additional FA-AML samples (3 FA-A patients ...
The leukemic stem cell (LSC) hypothesis postulates that leukemia are hierarchically organized and leukemic stem cells have the capacity to self-renew, give rise to more differentiated progeny and maintain the leukemia long-term [6–8]. CD34+CD38− cells have been shown to be able to initiate...
(PLT),初诊时骨髓白血病细胞平均 水平以及CD34,CD38和HLA-DR单个核细胞平均表达水平的关系.结果 该组病例 CD34,CD38,HLA-DR的阳性率分别为62.96%,82.72%和69.14%,各亚型阳性病例单个核细胞表达水平不一,HAL高表达 CD34和HAL-DR,M0较其他AML患者CD34和HAL-DR表达水平高,6例M3患者低表达CD34,CD38,不表达HLA-DR;...
中文摘要 急性白血病CDl33和CD34的表达及意义 摘 要 的表达及其与白血病类型、不同病期及预后之间的关系,进一步阐明其 意义。 方法:应用流式细胞术检测52例急性白血病和20例巨幼细胞性贫 18.0统计软件进行分析。 结果: (PO.01)。 2 31.3%和54.3%VS50.O%,无显著差异(PO.05)。 VS 37.5%,差异无显著性(PO...