During minimal residual disease monitoring, significantly higher levels of BCR/ABL transcripts were detected in subjects in the CD34+CD38−CD58− group than in other phenotype group, especially at 3 months post HSCT. In addition, CD34+CD38−CD58−LPCs are directly correlated with a higher...
Mayo Clinic, Scottsdale, AZ, USA and 3Flow Cytometry Core Laboratory, Memorial Sloan-Kettering Cancer Center, New York, NY, USA The most primitive hematopoietic stem cell (HSC)/progenitor cell (PC) population reported to date is characterized as being LinÀCD34 þ CD38ÀCD90 þ CD45R. ...
The CD34+CD38− cells lack expression of most lineage-specific antigens. This study was designed to test whether normal CD34+CD38− progenitor cells, free of leukemia, could be isolated by flow cytometry from children with B-lineage ALL. Only patients with B-cell precursor ALL whose ...
Cell sorting of CD34+/CD38+ cells from human BMSCs identify an osteoprogenitors that has the ability to differentiate into functional osteoblasts in culture (Chen et al., 1997). Additionally, CD200+ cells loaded on biphasic calcium phosphate (MBCP) ceramic disks and implanted subcutaneously in ...
(FI). The CD34−level of FI was determined based on the Fluorescence Minus One controls.dThe cells residing in the R3 gate were further subdivided into 18Lin−CD45+CD34+CD38−(R5) cells. The CD38−fraction contains cells expressing <10% of the maximum PE-Cy7 FI.eThe R5-gated ...
Here, we report that this rare population has the ability to self-renew and is more primitive than the Lin−CD34+CD38− (+/−) cell population, because it is able to generate self-renewing +/− cells in vivo and is more competent in serial transplants than the +/− cell ...
A novel Lin|[minus]|CD34|[plus]|CD38|[minus]| integrin |[alpha]|2|[minus]| bipotential megakaryocyte|[ndash]|erythrocyte progenitor population in the human bone marrowLeukemia is one of the leading journals in hematology and oncology. It is published monthly and covers all aspects of the ...
Cell supernatants were obtained from: different donors (A through G) and sources (UCB, BM, MPB) of CD34+ cells; sorted MPB CD34+/CD38+ cells; a human polyclonal T-cell line (PBL); the mouse stromal cell line MS-5. Supernatants were assayed for the presence of SDF-1 by an Ag ...
Lin−CD34+CD38+ progenitor cell-enriched cell fraction and Lin−CD34+CD38− integrin α2− cells gated for CD45RA expression. Shown are representative FACS plots of two separate experiments. Quadrant gates in the FACS plots were set on the basis of fluorescence minus one staining profiles...