Here, we focus on the importance in caution when interpreting MDR in AML. Bone marrow (BM) sample was assessed toFLT3andNPM1mutation,RUNX1/RUNX1T1andCBFB-MYH11rearrangements, karyotype (KT) and immunophenotyping. Using an 8-colour antibody panel (CD45, CD34, CD117, CD 33, HLA-DR, CD38...
engagement of checkpoint receptors) or soluble factor release (i.e., ROS, NO, and arginase I). Human neutrophilic MDSCs are known to upregulate arginase I to inhibit CD8+T cell activity13. We thus investigated whether CD8+T cell suppression was dependent on arginase I activity in CD45+CD33...
MDSCs (CD33+/CD11b+/CD14−/HLA-DRlo/−)* 0.1% ± 0.02% 6% ± 1% <.0001 SSC, side scatter. * Percent of all cells based on FSC/SSC gate excluding debris. † Percent of CD7+/CD56+/CD3− NK cell population. Activation of NK cells through CD16 is intact in MDS patients An...
Immature myeloid cells (HLA-DR−/lowCD33+CD16−) were tested before and after granulocyte colony-stimulating factor (G-CSF) administration in healthy donor and isolated for suppression assays and co-culture with T cells in vitro. Isolated cells were infused in humanized mice for a xenogeneic ...
(MDSCs) are broadly classified as either neutrophilic or MO MDSCs, and are phenotypically identified as being CD11b+CD15+CD66b+CD33+CD14−or CD11b+CD15−CD33+CD14+HLA-DR-/low, respectively5,6,7,8. In our previous studies, we observed a subset of immunosuppressive myeloid cells in ...