Here, we focus on the importance in caution when interpreting MDR in AML. Bone marrow (BM) sample was assessed toFLT3andNPM1mutation,RUNX1/RUNX1T1andCBFB-MYH11rearrangements, karyotype (KT) and immunophenotyping. Using an 8-colour antibody panel (CD45, CD34, CD117, CD 33, HLA-DR, CD38...
Additionally, IFN-γ-educated CD33+HLA-DRlow MDSCs had less capacity to suppress T cell proliferation and activation. Neither NO production nor ARG-I activity influenced the immuno-suppressive potency of IFN-γ-educated MDSCs. Suppressive function of cytokine-induced MDSCs depended on PD-1/PD-...
Immature myeloid cells (HLA-DR−/lowCD33+CD16−) were tested before and after granulocyte colony-stimulating factor (G-CSF) administration in healthy donor and isolated for suppression assays and co-culture with T cells in vitro. Isolated cells were infused in humanized mice for a xenogeneic ...
engagement of checkpoint receptors) or soluble factor release (i.e., ROS, NO, and arginase I). Human neutrophilic MDSCs are known to upregulate arginase I to inhibit CD8+T cell activity13. We thus investigated whether CD8+T cell suppression was dependent on arginase I activity in CD45+CD33...