特别注意巨噬细胞需在实验前48小时完成诱导分化,采用含100ng/mLPMA的培养基处理THP-1单核细胞系。备选方案建议准备三批次不同来源的CD206抗体做交叉验证,推荐购入商品化试剂盒时保留完整的质检报告。 实验材料要包含预冷的细胞固定液(建议采用含有1%多聚甲醛的PBS缓冲体系),破膜剂选择含0.1%皂苷的TritonX-100...
Western Blot analysis of CD206 (MMR) using CD206 (MMR) Monoclonal Antibody (15-2) (Product # MA5-28581) on lysates of differentiated monocytes (two batches), and on THP-1 and Raji cells (negative controls) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with ...
Western Blot analysis of CD206 (MMR) using CD206 (MMR) Monoclonal Antibody (15-2) (Product # MA5-28581) on lysates of differentiated monocytes (two batches), and on THP-1 and Raji cells (negative controls) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with ...
鉴定方法 M1、M2具有各自特征性标志分子，iNOS、CD86、TNF-α是常见的M1型巨噬细胞的标志分子，IL-10、Arg-1、CD206是常见的M2型巨噬细胞标志分子。一般在实验研究中：1 WB可以分析RAW264.7细胞iNOS、YM-1和Arg-1的表达水平（如图3）；图3. WB分析照射对IL-4诱导的RAW264.7细胞YM-1和Arg-1的表达影响 2...
由单核细胞系THP-1分化而来的M2型巨噬细胞通过HGF/c-Met信号通路在体外诱导肝癌对索拉非尼耐药。HGF还增加了单核细胞从血液中的募集,形成了一个前馈循环。在荷瘤小鼠中,M2型TAMs在索拉非尼耐药肿瘤中比索拉非尼敏感肿瘤中聚集更多,并产生大量HGF。这些新见解为cMET/HGF抑制剂(卡博替尼或特泊替尼)单独或联合...
Western Blot analysis of CD206 (MMR) using CD206 (MMR) Monoclonal Antibody (15-2) (Product # MA5-28581) on lysates of differentiated monocytes (two batches), and on THP-1 and Raji cells (negative controls) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with ...
The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 ...
Immunohistochemical analysis of paraffin-embedded MUTZ-3 cell pellet (left, positive) or THP-1 cell pellet (right, negative) using CD206/MRC1 (E2L9N) Rabbit mAb. Orders: 877-616-CELL (2355)•orders@cellsignal.com•Support: 877-678-TECH (8324)•info@cellsignal.com...
MSDS ¥2230/100tests Cat No.PE-65155 浓度:10 μl/test 库存:2-3周货期 收藏 点击咨询售前客服 点击咨询售后客服 humanFC Novel Collagen Analogs with Multicopy Mucin-Type Sequences for Multifunctional Enhancement Properties Using SUMO Fusion Tags ...
三,实验结果 1.体外诱导TAMs PMA及IL-4将THP-1诱导成为M2型巨噬细胞,WesternBlot检测巨噬细胞特异性标志物CD68及CD206阳性. 2.原代巨噬细胞的提取及鉴定 (1)... 梁浩 - 广州医科大学 被引量: 0发表: 2019年 M2型肿瘤相关巨噬细胞浸润与食管鳞癌发生,浸润及转移的关系 目的:探讨M2型肿瘤相关巨噬细胞(M2...