Doublets were excluded based on forward scatter (FSC) profiles, and dead cells and T cells were excluded by gating 7-aminoactinomycin D–negative (7-AAD−) and CD3− cells, respectively. B-cell subsets were defined as: B1 cells, CD19+CD20+CD27+CD43+CD38lo/int; naive B cells, CD19...
In conclusion, after treatment with rituximab there remains a B cell population with different functional capacities. Consequently, the effect of rituximab on the immune response will not only be determined by the extent of B cell depletion, but also by the functional properties of th...
在一些实施方案中,该治疗剂可包含针对CD3和CD38的双特异性抗体。在一些实施方案中,该治疗剂可包含针对CD3和gpA33的双特异性抗体。在一个实施方案中,该双特异性抗体是MGD007。在一些实施方案中,该治疗剂可包含针对CD3和CD20的双特异性抗体。在一个实施方案中,该双特异性抗体是/> 在一个实施方案中,该双特异性抗...
cd20在来自外周血或淋巴器官的大于90%的b细胞的表面上发现,和在早期前-b细胞发育期间表达,和保持存在,直至浆细胞分化。cd20存在于正常b细胞以及恶性b细胞二者上。特别是,cd20在大于90%的b细胞非霍奇金氏淋巴瘤(nhl)上表达(anderson et al. (1984) blood 63(6):1424-1433),但未在造血干细胞、前-b细胞、正常...
Human tonsillar PCs (CD19+, CD38bright), CD4+ T cells and B cells (CD19+, CD38lo, IgD+) were sorted to more than 90% purity and cultured at 50 000 per cell type per round-bottomed well in 200 µL RPMI/10% FCS/penicillin G (200 U/mL). Transwells (0.2-µm pore size, ...
更具体而言,本发明涉及通过向受试对象给予试剂的组合,抑制b细胞群增殖并治疗b细胞增殖性紊乱的方法和试剂盒,例如血液学癌症,其中所述的试剂的组合包含:(i)至少一种p13激酶(p13k)-δ的抑制剂;(ii)至少一种抗-cd20抗体;以及(iii)至少一种bruton酪氨酸激酶(btk)抑制剂。 背景技术: 尽管科学和临床的专项研究进行...