骨髓来源的抑制性细胞(Myeloid-derived suppressor cells，MDSCs)是骨髓来源的一群异质性细胞，是树突状细胞(dendritic cells，DCs)、巨噬细胞和(或)粒细胞的前体，具有显著抑制免疫细胞应答的能力。小鼠MDSCs的表型为CD11b+Gr-1+，分两个亚型:粒细胞样的MDSCs(CD11b+Ly6C-Ly6G+)和单核细胞样的MDSCs...
骨髓来源的抑制性细胞(Myeloid-derived suppres洋战尼行sor cells,MDSCs)是骨髓来源的一群异质性细胞,是树突状细胞(dendritic cells,DCs)、巨噬细胞和(或)粒细胞的前体,具有显著抑制免富烈绍学治盾济条免疫细胞应答的能力。 小鼠MDSCs的表型为CD11b+Gr-1+,分两个亚型:粒细胞样的MDSCs(CD11b+Ly6C-Ly6G+)和...
2012. CD11b + Ly6C ++ Ly6G − cells show distinct function in mice with chronic inflammation or tumor burden . BMC Immunol. 13 :69 10.1186/1471-2172-13-69 [ Cross Ref ]Kallberg E, Stenstrom M, Liberg D, Ivars F, Leanderson T (2012) CD11b+Ly6C++Ly6G- cells show distinct function...
To this end, we sorted CD11b+ cells using FACS with regard to the expression of the Ly6G and Ly6C markers into three populations; Ly6C+G+, Ly6C+G- and Ly6C++G-, as shown in Figure 1A. RNA was prepared from the isolated cell populations and S100A9 expression was measured using Q-...
CD11b(+)Ly6C(++)Ly6G(-) Cells with Suppressive Activity Towards T Cells Accumulate in Lungs of Influenza a Virus-Infected Mice. Almeida,T,etc.CD11b(+)Ly6C(++)Ly6G(-) Cells with Suppressive Activity Towards T Cells Accumulate in Lungs of Influenza a Virus-Infected Mice.[J]... P Milanez...
另一方面,糖代谢限制可以直接诱导Ly6GlowG-MDSC的凋亡,而不是Ly6Ghigh亚群的凋亡。总之,这些结果表明,禁食治疗诱导的糖代谢限制减弱了免疫抑制环境,增强了CD3+T细胞的激活,为加强基于免疫的癌症干预提供了潜在的解决方案。 Intermittent fast...
CD11b.sup.+.sup.Ly6C.sup.++.sup.Ly6G.sup.-.sup. cells show distinct function in mice with chronic inflammation or tumor burden Stenstrom M, Liberg D, Ivars F, Leanderson T (2012) CD11b+Ly6C++Ly6G- cells show distinct function in mice with chronic inflammation or tumor burden... E Ka...
为进一步明确具体哪一类免疫细胞参与此过程,作者应用流式细胞技术分析了Ang II灌注的动脉中炎症细胞,结果表明CD45+CD11b+Ly6C+Ly6G-F4/80+巨噬细胞呈现时间依赖性地增加。这些结果提示,骨髓巨噬细胞中CD11b的表达上调可能参与高血压的发生发...
在进行细胞表面分子染色前,用CD16/CD32抗体孵育细胞悬液封闭细胞表面的Fc受体。用于检测细胞表面蛋白的抗体包括:购自ebioscience的CD3e、CD4、CD8a、CD19、NK1.1、F4/80、CD45、CD11b、CD45.1、CD45.2、Ki67、Ly6C和购自biolegend的CD115、Ly6G、和Siglec-F。IL4Rα抗体购自R&D。
+ve cells, young as well as aged animals had ~50% Ly6G and Ly6C positive cells (Supplementary Fig. 3a). Furthermore, ~5% CD11c+CD11b+ cells of young and ~10% of aged animals were NK1.1 positive. Therefore, splenic CD19−veCD3ε...