G-MDSCs (CD11b+Ly6Cint/loLy6G+) share many phenotypic and functional features of neutrophils, whereas M-MDSCs (CD111b+Ly6C+Ly6G−) are related to monocytes [10, 11]. The granulocytic nature of CD11b+Gr-1+ cells has been detected in the lung tissue of mice with mammary adenocarcinoma...
BM monocytes (Ly6ChiLy6Gint) in E. coli-infected vagotomized and Chrna7−/−Itgam−/− mice were increased compared to E.coli-infected wildtype mice, whereas BM neutrophils (Ly6CintLy6Ghi) were not different among these three groups (Supplementary Figure S11C–E). These findings ...
Flow cytometry analysis of CD45intLy6CCX3CR1+ microglia, CD45hiLy6C+Ly6G monocytes, and CD45hiLy6C+Ly6G+ neutrophils revealed significantly reduced cell counts as well as reactive oxygen species (ROS) production in CD11b KO mice at d3 post-injury. Further examination with NanoString and RNA-...
Representative dot plots of three mice indicating CD11bLy6G þ and CD11bLy6C þ cells (d) and bar chart of 3–4 mice±s.d. Student's t-test significance: **P40.005. (f) Intestinal permeability as determined by quantifying the amount of fluorescein isothiocyanate (FITC)–dextran levels (...
Live cells were gated as follows: B cells (CD3-CD19 þ ), CD4 T cells (CD19 À CD3hiCD4 þ ), CD8 T cells (CD19 À CD3hiCD8 þ ), NKT cells (CD3int NK1.1int TCRb þ ), NK Cells (CD19 À CD3 À NK1.1hi), monocytes (CD19 À CD3 À Ly6c þ Ly6g ...
Among the CD11b + cells gated on F4/80 expression, Ly6GintLy6C − was the dominant population expanded by LPS treatment, with the Ly6GintLy6Clo popula- tion being the minor subset (right panel in Supplementary Figure S1 online). As inflammatory monocytes express Ly6C but do not express ...