小鼠肺组织经过dispase酶消化后,用荧光标记的F4/80、CD11b、CD11c 和 GR-1抗体染色。 下图中(A, B) F4/80+ 、CD11b low 、CD11c+是肺泡巨噬细胞,而 F4/80+ CD11b high CD11c low被分为CD11bhigh 巨噬细胞。(C) F4/80-/low CD11c high 是DCs,...
Transcriptional profiling identifies markers that define the CD103+CD11b+ DC lineage, including CD101, TREM1 and Siglec-F, and shows that the absence of CD103+CD11b+ DCs in CD11c-Cre.Tgfbr1 fl/fl mice reflects defective differentiation from CD103CD11b+ intermediaries, rather than an isolated...
Among the cells that were associated with parasites, 11.7% are CD11c+ cells. Therefore, 5 × 104 CD11c+ MLN DCs were injected intravenously to recipient naive mice, and 9 days after, these mice developed a parasitic process as assessed by the detection of parasites in their brain (78.1 ± ...
cells by flow cytometry, we gated for cDCs (CD45+CD11c+MHC-II+CD88–F4/80–Siglec-F–), excluding Siglec-F+alveolar macrophages (AMs), CD88+F4/80+IMs, F4/80+monocytes, and CD88+MHC-II–neutrophils (Supplementary Figs.1dand3a, b). At steady state, there were very few Ly-6C+antig...
CD11c+myeloid cells prevent T-cell-mediated colitis Although these findings implied that TGFβR1 signalling in T cells normally prevents inflammatory disease, previous work suggested that the wasting disease seen in CD11c-Cre-Tgfbr2fl/flmice was dependent on DCs26. To investigate whether this might...
Tumor-educated CD11c+/CD11bint/Gr-1-regulatory dendritic cells show a mutated pattern of trafficking molecules implicated in cytokine secretion.Dendritic cells (DCs) play a pivotal role in regulating innate and adaptive immunity and are ... G Chiaruttini,I Soncin,JL Stow,... - 《Cancer Resea...
CD31+/CD11b+/Gr-1+ precursors require IL-4, together with GM-CSF, to give rise to mature myeloid DCs (CD11bbright/CD11c+/CD8α−), and at least a part of these cells can arise from an intermediate stage of CD11bbright/CD11cdim/−/CD8α−adherent cells (Figure 7). Why pGM-...
3B). Out of all CD11c+ cells located in the PP SED region, 56% were classed as LysoDCs in naïve mice which was close to the 54% that was observed in CT + OVA immunized mice. A lower frequency of MerTK+lysozyme+ cells was observed in the TZ, with 21% in naïve and 26% in...
programgenerationofCD11c low CD11b high Ia low DCswithregulatoryfunction(CD11b high Ia low regulatoryDCs).However, whetherandhowthetumormicroenvironmentcanprogramgenerationofCD11b high Ia low regulatoryDCsremaintobe investigated.Inthisstudy,weusedthefreshlyisolatedtumorcellstomimictumormicroenvironmenttococulture...
Moreover, the expressions of myeloid differentiation primary response 88 (Myd88) and nuclear factor kappa B subunit 1 (Nfkb1) were higher in CD11chiCD11b+ DCs at the disease stage, leading to higher nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) p65 phosphorylation ...